FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7298418
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG182800.25046523780907043No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC170920.23418773767136933No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT151890.20811359393227408No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG132030.18090221743945056No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC124440.170502703462586No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT115420.1581438607654426No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG114060.1562804432412613No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA93670.12834288197798482No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG88970.12190313024000544No Hit
GTCTTAGGAGTCTCAATCTTAAATCTTGGGCAGAAGAGGTACACCAAAAC88660.12147838065728765No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG86700.11879286716655582No Hit
TTTCTAAACCATTCAGGTTGGTTCCTTGTGATGTATGTTATCATTGCTAG86190.11809408559498784No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA82460.11298338900293187No Hit
CTGTAAAGGAGAGCTCTGTGTCTTGTGAGTTAGTCATCATCTTTCTCACA78280.10725612043596298No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA77630.10636551647219986No Hit
CTGTAACTCCAATGCTCATGTCAGCCGATTCATTAATTCCGGAAACTCCA77210.10579004929561447No Hit
TTATAGGGGCAATGCTCAAGACATTTCTAAACCATTCAGGTTGGTTCCTT75320.10320044700098022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA31500.083.5762562
GGTACTG32200.081.262685
CAGGTAC40400.064.955313
GTACTGA41900.064.3536
AGGTACT47450.056.522744
ACTGATC60100.050.5338488
TATAGCG39700.048.228112
CGAAAGC17200.047.2038083
GCGAAAG17250.047.08862
GCGGTAT44300.042.541176
TATACTA18400.041.7806242
GGATAGC50300.041.5015535
TTTGGAC41000.041.1964233
GACAGTA43750.039.7629857
TACTGAT70000.039.1416857
AGTAGAA44700.038.486541
GTATCCC49800.038.4263659
CTACTAG20150.038.1303376
CGGTATC50500.037.6058777
TACTAGT21300.036.7527587
Produced by FastQC (version 0.11.5)