FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211362
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG70380.1671193309907816No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC67710.16077933932062835No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG65440.15538915913664034No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG64570.15332331915423086No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG63790.15147118675620858No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG59730.14183060017163093No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG52100.12371294607302816No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC47140.11193528364457864No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA47080.11179281192165386No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA45610.10830225470999644No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT43660.10367192371494069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG20050.054.5907485
GCAGGTA20300.053.2333682
TATAGCG9700.042.618372
AGGTACT27950.040.718314
GCGAAAG11350.039.6177252
GTACTGA33400.038.8479586
GACAGTA30050.038.3547747
GCGGTAT10650.038.1153646
TTTGGAC31300.036.1316873
TATACTA11650.036.1073652
TTGGACA36550.035.699084
CAGGTAC30950.035.369133
CGAAAGC13100.034.8640063
ACTGATC38900.034.101098
GGATAGC32750.033.6425365
TAACTAT10500.032.491041
TTTAAAC36150.031.6852283
AGTAGAA34600.030.8387131
ACTACGC950.00470154530.5626771
GTATAGG40750.029.568921
Produced by FastQC (version 0.11.5)