FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971433
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC31750.10685080228966966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG10200.030.5726282
AGCGAAA11100.028.1188561
CGAAAGC12350.025.2392163
GTGATCG4951.8189894E-1221.9668489
GCACCGT22650.021.4416926
TATACTG15850.020.5791955
TACACCG6550.019.9194055
AGCACCG22100.019.679055
ACCGTGT24500.018.9353648
CTAATAC14700.018.738753
ATAGCAC32150.018.2632683
CACCGTG29450.018.2143447
ATACTGT29150.018.1525146
GAGCGAA6102.1827873E-1117.8489881
CCGTGTT23650.017.7778229
GTATATA19600.017.7761361
ACTACGC2901.8501087E-417.520731
ACCGTGC4608.1223334E-817.3338348
TAGCACC30750.017.208284
TGATCCG4307.6255674E-716.8582789
Produced by FastQC (version 0.11.5)