FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294476
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC73940.13965499135325196No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG73750.13929612675550895No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA68310.12902126669381447No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC55050.10397629529343413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA38500.033.5647131
GTGATCG6750.033.290539
TATACTG30550.032.743925
ACATGGT47850.026.5105635
GTAATAT29550.026.2875421
GACATGG51600.024.3031924
GGTCCTA64800.024.1624839
TAGCATG70550.023.440082
AGCGAAA20800.023.384881
GTATTAT58400.023.3705351
ACGAACC18250.023.0371065
GCATGTT72000.022.6525424
TGACATG59450.022.3170033
GCGATTA3254.001413E-822.305939145
GCGAAAG23400.021.3872282
GTCCTAT65450.020.9640791
AGCATGT80450.020.2765063
TAATATA38650.020.2672822
GTATAAG23350.020.2092481
CGAACCC21250.020.1261186
Produced by FastQC (version 0.11.5)