FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1793_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGCCCCTTCTCCACCATCGTCATTGTTTAATAAAAAGTTTTTTTG13.0303030303030303No Hit
GCTCCCTACAACAGCCACATCCACAGCTGAGCAAAAACGGAAAATCTTTC13.0303030303030303No Hit
CCACAAGACAAGTCAATAAATTCCAGAAGCCTAGAATTTTCCCAAATGAG13.0303030303030303No Hit
CTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCCCAGGGGCGA13.0303030303030303No Hit
TCACTGTACATTTTCCTCAACTTCTTTTATTTTACAGAGGTAGAGAGGGC13.0303030303030303No Hit
GTTACATTCATGAAATAACATAATGGCATACAAAAGACTGATCAAGAGAT13.0303030303030303No Hit
GTCGTAAGAGCCGTTGCATATCACTTGTGGCAGGGCACCTCTCGCGCAGT13.0303030303030303No Hit
TCATTATATATCTGGATTATTTCCAAATAAGATAAAATACTGAACCATTA13.0303030303030303No Hit
CTAAAACAGTGCACAGCTGATTTCCCACACAAACCAACTGGCCATGATAG13.0303030303030303No Hit
ACATTTAACAAGTCAATTTTCAATTCGGTATATTGTACTTGACCCCACCA13.0303030303030303No Hit
TTGTGCTTGAGATTGGTGTTGGAAAAATGAATTTAAAAAGCATTAAACAA13.0303030303030303No Hit
AAGCAGGGCCCTATCCCTGGCCCTGGGAGCATGCCCCGAGGCCAAAGGCA13.0303030303030303No Hit
GGCTCTGTGTGGCGGTGTTGGCGACTGGTGTGGGGGGTGGGCTGTCTATG13.0303030303030303No Hit
GTATATTATAAAGCAAGTATCAAAGCTTGGGTCTCAAGACTCTATTACCA13.0303030303030303No Hit
TTGTCTCTGTCTGTGAGAAGCACATATTCGTTAACCATAAAAGAGGATGA13.0303030303030303No Hit
NCCCACTTGAGTGGCTAATGGTCTTCTCCAGCAAGTCACATAGCTACTGA13.0303030303030303No Hit
CCAGGAACCTCATGAAGGGCTCAATTCCAGGACCCTAAATTCCTGCCTTG13.0303030303030303No Hit
ACGGGAGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCCGCAAGC13.0303030303030303No Hit
AAGAAATCCGGCAAGGGCTATCAGAATCTCTTAGCGCAACTAGAGGGTGG13.0303030303030303No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT13.0303030303030303No Hit
ATCTAGCACTGTTCAGGGGATTGCAAGATTGAAGATCTTATTATGTGAAT13.0303030303030303No Hit
GAATCAACTCGGTGATTGCAACATTGGGTACGCTTTATGTTGCAAGAGGA13.0303030303030303No Hit
CCCGCCTCCTGTGCGAAGAGACGGAAGCGGGGCTGCCAAGGAGACAGGAG13.0303030303030303No Hit
TAGTGAAGCTTTCTCTACCAAGCCTATCTAAAATATCTCCCCATCCCATT13.0303030303030303No Hit
ACTATGAACAATTACACCCCAACAAGCTGTATAATCCAAAAGAAAAGGGT13.0303030303030303No Hit
GTTCAGATGTCTTTGGCTTTAGGATTCAGTCTGAGTAGGTTGGAGGGTTA13.0303030303030303No Hit
AGTGGGTAGAGAGATTGAGTGAGAGAAAATTCTGTGTAATCAGGATTAAG13.0303030303030303No Hit
CAACCATACTAACCTTTGTTTCAAGGCTTACCTCCTCTCGCTCTTCAGCA13.0303030303030303No Hit
CAAAACGCGCTGGGGCTGGGACCCGACCCCCTCTGACAGAGTCTAATCCC13.0303030303030303No Hit
TGGCAGCTCAGTGGGTTGTGACTCTTGCCCTCAGTGTTGTGAGTTAGAGC13.0303030303030303No Hit
GGCTTAACCACAGGGAGCCACAGAGATCTGTCATGAAGATTCCTGGCTTG13.0303030303030303No Hit
AGCGAAAGCAGGAGGCTGTCAAGCTCACTGCAGATGAACTGGGAAACTCC13.0303030303030303No Hit
CACGACCCTGAGATCATGACCTGAGCCGAAGTCCAGAGTCAGATGCTCAA13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)