FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172859
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20200.035.5802231
GCGAAAG22600.031.4519422
AGTACGG2903.965397E-1027.495665
CGAAAGC27950.026.4578783
TATACTG29500.021.6237055
TGTCGCG2053.671531E-421.2176145
GTCCTAT31550.020.9395261
GCGTATA3602.587367E-618.1495081
TCTATAC52200.016.8055023
CCTATCG3052.7474636E-416.637049
CGACGAA3554.7088688E-516.336555145
GTACGGA5152.937977E-715.4829946
GTTCTAT55950.015.3111131
GTATATA31900.015.2478621
GTGTATA36700.015.231741
AGCGTGT11300.014.7543098
GTACTAG13400.014.627961
CCCTACG12300.014.1441098
CTATACC57000.014.116334
TAGTACT17500.014.0836284
Produced by FastQC (version 0.11.5)