FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAGTCATTTCACTTCTGGTATCATGGGCACTGGGGGTTTTGAGGAG15.88235294117647No Hit
TTCATCAACATCGATAAGAACCACCTCGAAGACGTGCACTCGGCCCATCC15.88235294117647No Hit
CTCTCACACACACCCACAAACACACCTTCTTCTCGAACCTCTATCACATA15.88235294117647No Hit
GCCTAGAATATCTTGTTGCTTCTTCCGCAAGCAGGTGTAGTGCGCATGAG15.88235294117647No Hit
CCCCCCATGACATCCCACTCTTAACAACTCTCTCTGGTACCATGCATACC15.88235294117647No Hit
AGCGCGACCTCATCAACGAAACACTGTTTCAGAACGGTGTACTATGTACC15.88235294117647No Hit
CATTACCAAAAGGGAGCTCTGAATTTAGAGAACCCAAATCTATCATAAAG15.88235294117647No Hit
CTCCTAATCAATGTCCTTGCCTCTGTCTTGAACTCCACACAGCCACCCTG15.88235294117647No Hit
TCACACTACAGGGCACTCCGAGGGTCAATCCCTTGGTTTACACTAAACCA15.88235294117647No Hit
ATGCTGCTCTGGCTCATTCTCTCTCTCTGTTTCTCCAGACCCCTCTGAAG15.88235294117647No Hit
AAACAGTAGTGACTCTAGTTATAGAGGAATTGAGGTAACTGTCAACGCAT15.88235294117647No Hit
GATCAGCATTCTCATGCACAAGTCACAGTTAGTACATGTAATAGAAAAAT15.88235294117647No Hit
GAGTTGGTTTCTCAGTCCTGTGGCAAGACCAGAAATTGTAGTTTCTGACA15.88235294117647No Hit
GTTCTAGGGCCAATTGAGAGGAGTATATTCCTTTAGATGCTTTTGGATGG15.88235294117647No Hit
AACTTGGGGGGAGGGATTCGAAAGAGGGAGGCAGAGCCAATTACCGCTTT15.88235294117647No Hit
GAAAAAGCAGTACGTATTCATATGTCAAAATCGCATCAATCTATCTCCCA15.88235294117647No Hit
GCTCTAGATAGTACACAGAGGAAAACCAGCTAGAAATGCGAGTGTGGGAA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)