FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329401
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC5000.037.697197145
GCGAAAG15150.034.47082
CGAAAGC16050.033.4254653
TATACTG23150.031.3126355
TATAGCG4900.029.6050242
GTGATCG8350.029.5167169
CGCTATA4250.027.3062782
AGCGAAA20550.025.7865941
TATACCG11100.024.8159665
GCACCGT28800.021.3942496
GCGGTAT6850.021.1645976
AGCACCG28850.020.6033865
CTATAGG14550.020.4552041
GTATAGG17250.020.1992421
ATACCGA12600.020.1357636
GTACCGA11950.020.0178137
TATACTA12750.019.898875
GTCGGTA11800.019.659555145
TACCGAG12950.019.5915537
TCGCCAT18600.018.708284145
Produced by FastQC (version 0.11.5)