FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909058
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT45170.11555213557844371No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42120.10774974431180095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG3200.049.8662262
GCGGTAT4550.033.456076
GTTACGT4250.032.4458961
GTATAGG17950.029.11141
TATACTG29050.028.6958275
GCGAAAG17050.028.502582
TAATCGT1804.710544E-628.1898389
TTACGTG4900.028.124922
AGCGAAA18350.026.4992641
CGCGTCG1100.00967723926.362364145
GGATAGC13150.025.3570755
TGACGCG1450.001227427424.998793145
CGCTATA2401.5938022E-624.1775632
CGAAAGC19800.024.1648853
GTCGGTA10700.023.036274145
TTATACT29700.022.6997624
CGGTATC6900.022.0616117
TACGTGT6300.021.8634643
TAGCGGT7800.020.4466864
CAGTATG20750.020.261759
Produced by FastQC (version 0.11.5)