FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGAGATGGCATCATCAAGTGAGCATGAAGAAGAACAAAACGAAGTCG15.88235294117647No Hit
GGACTCAGGGATCATAACCTGAGCAAAACTCAGAAGCTTAACCAACAGAC15.88235294117647No Hit
ATCTTCCCCGGAGTCACATAACCTTAGCTCAAGTGGATCAACCAGTGACG15.88235294117647No Hit
GAAACAAACAAGCGAGAGAGAGAGCAAGCTGACCCGAGAGCCCAATCTGC15.88235294117647No Hit
GAACATATCTAATAACCAATCTAGTAGTGAGGGATGGACTTACTGCAAAA15.88235294117647No Hit
CCCCTGCCGTGCCTGCCCAGGCAGGACAACACCAGCCACCCTCGCACCCG15.88235294117647No Hit
ACACTTATGTTACAAGCTGTAATCATTTTCCTGATTTCTATGTTCCGTGG15.88235294117647No Hit
GTGAGGTATCCAAGGTATCACCTGATAAGCGCGCATGGCATGGACAGCCA15.88235294117647No Hit
CCCCCCTGGTAGGCCCTTGTCATAAGTGCACCCGGAAAACTCGGAGCGAG15.88235294117647No Hit
CTCTATGCCCAGTGTGTCGTTGAAACTCAAAACCACAAGATCAGGAGTCA15.88235294117647No Hit
TATTTAAATATACAATTTCAAAGACGAATGTTGGAAATGATGCATAATGA15.88235294117647No Hit
CGTCTGAGGATGTAGAAGCATGTGGAGACAAGGCACCAGCAGCACCGCTG15.88235294117647No Hit
GTTTATAGTTATACAGCCCTAAATCAAGAAACAAGAAACAACTCAAATAA15.88235294117647No Hit
TCGGTGATGATGGTCTGCGCCTGTGGGCTGTAGAGCCAGTTCAGCTAGGC15.88235294117647No Hit
GAGGAGGGAACTGGGGAGCTATTCAGTGGGTCCAGTGTTTCCAATGAAAC15.88235294117647No Hit
TTACTAAATAGACCAGTTACAAACAGATTGGTCAGATGGTGATACCTCTT15.88235294117647No Hit
CCTTACAACGCCGAAGGTGTGTTGGCGGATGAGAGAAAGATTTTCCGCCT15.88235294117647No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)