FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1793_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158197
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC81850.15867947656904147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19250.036.171012
AGCGAAA22250.031.6454771
TATAGCG7300.030.8005852
CGTACGG1504.4082582E-528.9941625
GGTACTG26450.027.678745
GTACTGA32150.026.3788286
ACGTACG1400.001001086125.888154
TCTATAC56100.025.7147853
CGAAAGC28250.025.1478183
TATACTG31800.024.8456295
TATACCA65350.023.6256985
AGGTACT33200.023.3617134
ACTGATC39100.023.172868
GACAGTA33750.022.9807837
GTTCTAT65800.022.8357031
CTATACC65300.022.7561994
CAGGTAC33200.022.2717723
GCGGTAT10650.021.099046
GTTACGT4702.3464963E-1020.0777681
TACCACT69050.019.2106577
Produced by FastQC (version 0.11.5)