FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1793_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1793_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG21.1904761904761905No Hit
ATCCAATTCAAAGGATGAGATACAGACTTTACTTTTCAAAAAAGTGCCTG10.5952380952380952No Hit
TTTTTAAACAATTCGACACTAATTGATGTCCATCCGAATACTTTTGGTCG10.5952380952380952No Hit
TAACAATTGATCCTCCTATTCTACCACTGCCTAATACTTTTTTACCTGCA10.5952380952380952No Hit
CTGCATGTGCAGTCCCCTCCGTTTCGGGGATGCCGGCAATTTTTCAATAT10.5952380952380952No Hit
CGTGGTGAATAAGTACTTGGAAAAGCAAAGCGTAACATCAATCTTTGATG10.5952380952380952No Hit
GTACTGATCCAAAAAGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA10.5952380952380952No Hit
TCCCTAAACCACCCCAGTTCCCTTCAGCACCCGCTTAAGAGGCCGCCATC10.5952380952380952No Hit
AAATAGAATTAGCCATATGATCCAGCAATTGCAATTACTGGGTGTTTACT10.5952380952380952No Hit
AACCCAGGTCGTTTTTCAACAATTCGAGACTACTTGCTGGCCCTCCGATT10.5952380952380952No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTATATGGGGGATCTCCAGTATAAG10.5952380952380952No Hit
ACAAGTAATAAAGAATGTTAACCATCTCATAATGCTAAAAGAGTGTTTTT10.5952380952380952No Hit
GACCTGGGTGAGGCGCCCTTGGTTGAACGGCGGCGCCGAGATCAGCACTC10.5952380952380952No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG10.5952380952380952No Hit
CTCTGAGAATGAAGGGCCCCGGCAGTAAGGGGGTAACCCATCATATTGAA10.5952380952380952No Hit
ATTAGATTCCCATTGGATCGTACTCGCAATGTTTGGCCTGGTTTTAGTAC10.5952380952380952No Hit
GAGCAATAGTGGGCGAAATCTCACCATTACCTTCCCTTCCAGGACATACT10.5952380952380952No Hit
AGCAGGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAG10.5952380952380952No Hit
CATATGATCCACAGTGGTTTTTGTCAGTATCTCGCGAGTGCGAGATTGCG10.5952380952380952No Hit
GACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAA10.5952380952380952No Hit
GTTTAACCAGAGGTTGGATTAAATGAAATCAAGTGCAAGAGCAATGGGCT10.5952380952380952No Hit
GATAAAGAAGTTCTGGAGATGAATGGTGGTATGGTGGCAAAACAATGCGA10.5952380952380952No Hit
TAATTAGATAGTCGAAAAGCAATTTAGAGAATAACAAACATTTTTCATTA10.5952380952380952No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA10.5952380952380952No Hit
GTATTAGTCAACCTGTGCTAACATAACCAACCACAACAGATTGAGTTGCT10.5952380952380952No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA10.5952380952380952No Hit
GAAACGGAAACGGGACTCTAGCATACTTACTGACAGCCAGACAGCGACCA10.5952380952380952No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10.5952380952380952No Hit
CAGTTTTTTGGTAGCGGCGCTATCAAATGCTTTTTCTACATCTGTTGACA10.5952380952380952No Hit
GCAGTATTCAATGGACATCACATTGGTGGGTGTGTGTTTGATGATTTGAA10.5952380952380952No Hit
TCAAAGGGATGGTCAGAGATATAGTGAGAGCGCTGCTAGGTGTTCAAGTG10.5952380952380952No Hit
AAGAGATACAGGCCTAACTCAAGAAACAAGAAACATCTCAAATAAGCAAC10.5952380952380952No Hit
CCTCCAGGCTTTGGCCAGATGCCACCTTCCTGTCCTTTGCCACCTGGCTG10.5952380952380952No Hit
GCTCTAGTCCAGGGAGGAGAAACGTCAGGATTCTGGCGGCTCAGGCCAGA10.5952380952380952No Hit
CTCTTGGATATGTTGCCCTGTGCACATTCAACCACACTGGCCATCAGAGC10.5952380952380952No Hit
GAGCTCCCCCGACCCAGGTGCACCAGACTAGGACACTTGCGACCAAGTTT10.5952380952380952No Hit
AATTATGGGAGTTGAAAAGCAAATGGAGGGTGTCTTATGAGATTAGGGGA10.5952380952380952No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCCCCAGCATTAA10.5952380952380952No Hit
GCCAAGGGCAACTAGCTTACTTCCCGTTATCAAAGATTTGGGTTCTCTAA10.5952380952380952No Hit
AATCTATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCT10.5952380952380952No Hit
CCCTCTCACGGTCTGGGTGGAGGCTTGATTGAGCTGCTCTCTCAGAGCCT10.5952380952380952No Hit
GATTAGTATGCTTATGTTTAAAAGTATATTTTATTGTGTTAAATTGAATA10.5952380952380952No Hit
TACCAGGACAACGTGATCATGACATGATAAAAATTCCTAAAAGTAAAAAC10.5952380952380952No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC10.5952380952380952No Hit
GAGATAACAAGTACGCAGAGAAGCACGCAGAGAAAGAGAGAGATATTATA10.5952380952380952No Hit
GGAAAGAACCCATGAAGAAAAGAAGGCAGGATTCCCCCAGAACTGATGAA10.5952380952380952No Hit
GCGCAAGGAGACGTAGTGTTGGTAATGAAACTGAAACGGGACTCTAGAAT10.5952380952380952No Hit
TTCGTAAGATTCCACATTTCATTAATACTTTTTTCAGGTCCATAACAAGT10.5952380952380952No Hit
GGAGAGATGAGGGCAGGAAGTTCTCAGTGTGCCAGATTGTATCTGTTCTT10.5952380952380952No Hit
CAATATAGCAGAATACCCAGACCAGTATTCACTGTCAACTATCACGTCCC10.5952380952380952No Hit
AAGTAAAGAAGCTTTCTGGGAATTGGAGTGGTAGCAATGAAGAGCCTTCC10.5952380952380952No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA10.5952380952380952No Hit
AGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACA10.5952380952380952No Hit
CTGACAAAAAACACTGTGGATCATATGGCCATAATTAACTAGTAAACATC10.5952380952380952No Hit
CTTCTCATACAACACTGTCCACCGGGAAGAATAGGGACTGCTTCTAGTAA10.5952380952380952No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG10.5952380952380952No Hit
GATGTGGACCCCAATTGCATTTTTGACATCCTCGTCAGTGTGTCCTGGAA10.5952380952380952No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCTGTCTCT10.5952380952380952No Hit
CAAGACCCTGGGATGATGACCCGAGACGAAGGCAGACACTTAACCAACTG10.5952380952380952No Hit
TTTCTTATGTACAAATTTGTTTGCTCGGTATAGGTGGGTGGGTGATGTAT10.5952380952380952No Hit
CCAAGATATCAATTGAAAAGGAACCGGATCCAAAATGGACCAATGAAAAA10.5952380952380952No Hit
CTATTGAGAGGAGGTATCCTAAGCGGCCACGCAGAACACCTCGCGGGCCA10.5952380952380952No Hit
ATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCTATT10.5952380952380952No Hit
AGTAGAGTCTGGAGGTTTGAGAGGATTTCAACTCATCGGCAATGAAGCCA10.5952380952380952No Hit
GTTTCCGTTTCATTACCAACACTACGTCTCCTTGCCCTATCAATACATTA10.5952380952380952No Hit
GCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATT10.5952380952380952No Hit
AACCTGGATAACAACCGGATTCGCAAGATAGACCAGAAGGTTCTGGAGAA10.5952380952380952No Hit
CAGTAGATATTTGTTTACAGTATGCTTCATTGACAGCACACCATCCACTA10.5952380952380952No Hit
TGAGCTTGGTGTGCAGAATGCCCAAGAACGCGGGTGACCAAGAGGAAGGA10.5952380952380952No Hit
GTATGTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCC10.5952380952380952No Hit
CAAAAAAAAATCGTTAAACACCATTATTATAGTGTCGTTGATACAATAAC10.5952380952380952No Hit
GTGAAGGCAAGCATATAGTGGAGCGGAGTCTGGAGGTAGAATCGGACGAG10.5952380952380952No Hit
AGGATACCCTCTCTCCCTCTGACCCTTCCTTCTCTAGCATATTATTGTGC10.5952380952380952No Hit
CACATAGAAGACAGCATCATGTAATCAGACTACCATATCATTCTCTTCAA10.5952380952380952No Hit
GCTGGGCGGCGCCGCGGGGGCGGGGGGCGAGTATGAGCAGGGGCTAGAAG10.5952380952380952No Hit
ATTCAGTATTAATTGATGGCCATGCGAATTCTTTTGGTCGCTGTCTGGCT10.5952380952380952No Hit
AGCACGTACTGATCCAAAAAGGAAGACTTTGAGCGACAGTGCTTTAATCC10.5952380952380952No Hit
TCAGAAGGGAATGTGGTACGTCTTTCCTGTTGCTCTTGCAAGGGTAATAC10.5952380952380952No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC10.5952380952380952No Hit
CATTTTTTCCTTTACGGGTCCAGTTCCTGCTGGTGGGGCCCAGTTAGCTT10.5952380952380952No Hit
GTTTTCATGAACATATTCTGGTTAATTCCTTTCAGTTCTGAATGTGCCGC10.5952380952380952No Hit
GACTTAGCGCGAGAGTGAAGCTGGACTGGAAGAAAATGTCAAAGTAAAAA10.5952380952380952No Hit
GAACTTCCCTCAGTGTCACACCAATTTCAGTGAATCGGTTTTCCTTGTAG10.5952380952380952No Hit
CTCTTACTTATTGAATTTTTAGCTCGCACTTTTAAGAAAAGAAAAGTCGG10.5952380952380952No Hit
AGGTTGCCAGGTGGGGGGAGAGGTGGGTGGGTGTGATGACCCTCGGGGGG10.5952380952380952No Hit
ATGAAGGACAAGCCAAATTCACTTCCATTGCCGTCCGAGCTCTTCAATGG10.5952380952380952No Hit
CCAATTAACATTGATTAAGGGTTTCCAGGTACTTGGAAAGTACAACTCTG10.5952380952380952No Hit
GACCGTAGCATACTACCTCTCTTATGTACCAGCATGAAGCCTCCATGCAC10.5952380952380952No Hit
AGATCTAACATGCCTCAAATTCCTTATAAAATTGAAATGAACGTTGGGGT10.5952380952380952No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTT10.5952380952380952No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGACAGGCAAATTCTGTTTTAT10.5952380952380952No Hit
ATACAAACCACAGTGTGGTATAATAATGCTTTCTGGAAGCTGGAAAACTC10.5952380952380952No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA10.5952380952380952No Hit
ACTGTATGAATGCTTTCAAAAACAGACATAAAATAACAATTATTGACAAC10.5952380952380952No Hit
CCATGGTTTCACCTGTGAATTCTACGAATCATCCACAGAAAAAAATGAAT10.5952380952380952No Hit
AAACAAGGTCGTTTTTAACCAATTCGACCCTAATTGATGGCCATCCGCAT10.5952380952380952No Hit
GTGTTTGTTGTCAACCTCCCTTTTTCTGAATATTGATGTGTTCTGTTGAA10.5952380952380952No Hit
GTCCTCTTGGAGTATGTTTGTTTATACGACAAGACGGGAAAATATGTTAT10.5952380952380952No Hit
GACTAGAACAAAACACACACACACACACACACACACACACGCACACTCTC10.5952380952380952No Hit
GAATTCCAAATCACAGAAGTAGCACGCATTCGCATAGCTAACATGGGAAG10.5952380952380952No Hit
ATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATGGAAGGG10.5952380952380952No Hit
GGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGCCCGCCTGTGGTAGGTC10.5952380952380952No Hit
GTTCTAAGCATTGCAATAAGGTTATTTGCTCAAACCTATTCTCTCTAATT10.5952380952380952No Hit
GTGAAGGAGCGGAACAAAAATTGACACGGGGACAGGAGGCAGCTGAGTCA10.5952380952380952No Hit
CCCGAATGAGGGCTAGCGCTGCAGGGCCGGGTTCCCGCGCGGACCCGGGG10.5952380952380952No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA10.5952380952380952No Hit
GCCCTGGAAGCTTCCTGGGGACCACACCCATTGAGGGCAATGCCACACAG10.5952380952380952No Hit
TAGCAAGGCTTTCCTAACAATAAGAAGCAAGTGGGAGAGGTAGTAAGTAG10.5952380952380952No Hit
GATATGACTTTATCGCACGCCACGAGGAAGAGAACAGGCTTTTAGGGTCT10.5952380952380952No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG10.5952380952380952No Hit
GAGTAAAGCTAGAATACTTCACGAGCAATGCCGTGGCATGATGCATTTAT10.5952380952380952No Hit
AGCAGGTACTCAACCAAAATCGAAGACTTTGTGCGACAGTGCTTCAATCC10.5952380952380952No Hit
GGCCTGGACTCATCCCCATAAGAACGCCCCTCGACGTACCGAGTCCTCAA10.5952380952380952No Hit
GAATTATTCTGTACAAACACCGCAACACTTAAGGAGTATGATGCAAATAA10.5952380952380952No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTAGCGCTGGCACTTTTAAGAA10.5952380952380952No Hit
TAATTCTACACTCATAAAATCAAATTAATGAAGAGCTAAAAGAATATATA10.5952380952380952No Hit
GATCAAAAGCTTTTGCACAGCAAAGGAAACAGTTAAGAAAACCAAAAGAC10.5952380952380952No Hit
CCCCATGATAGAGTAGGGAACTGCCACGCATAAAATTAAGCGAAAGGCCA10.5952380952380952No Hit
CACAAAAACACAAAATCTCATCATCCAACAAAAAAAGATATGTTTATTTT10.5952380952380952No Hit
AATTCTATCAGGGACAGTGATCGACTAAGAAACATCTGGGACACAGCTAT10.5952380952380952No Hit
TCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACC10.5952380952380952No Hit
CCCTTATACTGGCGCGCCCCCAGCTCGCCAGGGACCAGCCACAGGAACAC10.5952380952380952No Hit
CATACATCTTAGAACTGTGAATACCCACTTGCTTTACCGTCACTCTATCT10.5952380952380952No Hit
GCTAAGGGTAACCTTTGCCAGCTGATACCTGAAGAGATATGTCACACCAG10.5952380952380952No Hit
CAGTAAGTATGCTAGAGTCCCGGTTCCGGTTGATTACCAACACTACGTCT10.5952380952380952No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA10.5952380952380952No Hit
CAGCAAGCAATGCAGATAAAATCTGCATCGGCCACCAGTCAACAAACTCC10.5952380952380952No Hit
TTACTGCTGAAGTCCTAAAGTCTGCACTGCCACCCCTTCCCCATCAATAG10.5952380952380952No Hit
CCTGTCACTGGAGGGGTTCATTAGCAGGACCCAGACGAAAGTGCAATGGA10.5952380952380952No Hit
GAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAA10.5952380952380952No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.5952380952380952No Hit
CATTGGGATATTGCACTTGATATTGTGGATTCTTGATCGTCTTTTCTTCA10.5952380952380952No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCCTGTCTCTTA10.5952380952380952No Hit
CACTTGCACATCACAGCTTAGAGTACTCAGTGATTTTTTGAAAAAAAAAA10.5952380952380952No Hit
GTTTGGTTACCTATTTCTAGCAAAGCGTCTACTTTAAAAGACGATGTGAG10.5952380952380952No Hit
TCCCTAAGATTAAACCACGCAGCATAGACAACACACATGCTCCAGGCTCC10.5952380952380952No Hit
GTCCATTAGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTT10.5952380952380952No Hit
ATCTCAGTGACTACCCCTGATTCCGCATCGATCAGGACATGGCGATCTTT10.5952380952380952No Hit
ATTCATTATTGTTCCGTCTTAAGTAGGGACATATTAGACCCAGTGTTGGT10.5952380952380952No Hit
GTTTTTTACTTCTTATATGGCCTCTAACACATTCATTATTGTCTTGTGAG10.5952380952380952No Hit
ATAGAAGCATGGGACTTGGCCATGCAGAAAGTGGTTGGAGATCATGCATT10.5952380952380952No Hit
ATCATGAATTCAGTGAGGTTGAAACTAGACTCAATATGATCAATAATAAG10.5952380952380952No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG10.5952380952380952No Hit
GACCATATCCTGACTACAATACGAGGATGTGTAAAAAAGTGATTTATAAA10.5952380952380952No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT10.5952380952380952No Hit
CAACATGAGCAAAAAGAAGTCTGACATAAATCGTACAGGAACATTTGAGT10.5952380952380952No Hit
GGATTACCCAATGGACCTACCCAGGACCAGCATCATCCTTTTCCTCCCTT10.5952380952380952No Hit
GCAAATCTCCAAGGAATTTCACCAGTATCGAGAAAACAAAATCTGTTACT10.5952380952380952No Hit
CTTTAGGTAACATTTCAGTATGATAAACAATAAGACCAATATAGTTTTTT10.5952380952380952No Hit
ATCGTGAAGATCTGGGCCACCATTGAAGAGCTCGGACGGCAAGGGAAGTG10.5952380952380952No Hit
CTTTAATACCTCACACTTCCCTCAATGATCCCCCATCCAGACAGAAGATC10.5952380952380952No Hit
GAACATAACGCACTCAATGACAATCCACCAAAACAAGAGGGAAAACATCC10.5952380952380952No Hit
GTCTTGTGTTCTAGCCTTTGCAATCGAATTGCCAGGAATAGCTCATAGGC10.5952380952380952No Hit
GCATTAAGAATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAATCTAA10.5952380952380952No Hit
TCCCTTCAGTGCTAAGGAGTTTTAGGGGTAGACACTTATTAATGATCTTT10.5952380952380952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGG10.5952380952380952No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10.5952380952380952No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG10.5952380952380952No Hit
ACACCAATCACATTGGATTAGGGCCCACCCAATGACCTCATTTTAACTTA10.5952380952380952No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGAATTGCCACACCTATTTTAG10.5952380952380952No Hit
GCAATACTGCGCGAAATCAGACCATTACCAACCCTTCCAGGACATACTGA10.5952380952380952No Hit
TTCTTAGACCATGAACCAATACTGTCGGTAAGCATCCCATCATAAATGAT10.5952380952380952No Hit
CAGGAGGGAGAGCAAATAGTAGCATTGAACCCCCGCGGTTAGTGCAGGTG10.5952380952380952No Hit
TATCCCAGAAAACAACACAAGATTCTCCTGTCCAGACAGTATACACATCA10.5952380952380952No Hit
GTGATATCTGATTGTGGGTTTGATTTGTATTTCCCTGATGCCGAGTGATA10.5952380952380952No Hit
CACTAAGAGAAATGACTCTTGATGAAAAGTAAAGGGATATGTTAATTCTC10.5952380952380952No Hit
GGATATACATTACTCTGGAAATTAAAGACAGCTGCTAAAAGATCTTGGGG10.5952380952380952No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers