FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683476
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC44450.12067405895952628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17900.027.5533352
AGCGAAA20700.024.1987231
CGAAAGC21100.023.7071063
GCCGTAA1750.003661843720.713041145
TATACTG23400.019.5159385
GACCGTC3351.317987E-619.4751197
CCGTATA4202.8649083E-818.986954145
GTCCTAT33250.018.3400841
GCCGACC8850.018.021517145
GTATAAG12950.017.3782331
TATACAT27800.017.2093265
TCTATAC61550.017.0785833
ACGATGT5552.1864253E-916.9798098
CCGTCTA3504.1705447E-516.5695329
CGATGTA3103.1092454E-416.3690959
GTATATA34400.016.2497231
GTTCTAT57500.016.1605381
ATACCGT4501.226601E-616.1086126
TATAATA29600.015.927222
GTATTAT29650.015.9148431
Produced by FastQC (version 0.11.5)