FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4960483
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG23400.035.644022
CGAAAGC26450.031.5176263
AGCGAAA26700.031.2675911
CCGTATA2303.1479405E-522.062782145
GTCCTAT32350.021.542931
TATAATA44200.021.1676242
GTATTAT42650.021.106221
GTATAGG13150.020.4260221
ATTATAC21550.019.8465983
TATACTG22050.019.7241235
CGTATCG2256.8803685E-419.3302279
GTATAAG9800.018.519181
GTATAAT28800.018.1487981
TAAGGCG2000.00792910518.1215385
GTGTAAG39750.018.0803131
CTATGAC25200.017.8349743
GTATATA26350.017.6322291
GTTATAC12150.016.7056393
GTCTATG30450.016.6885491
TACTATA17900.016.6125182
Produced by FastQC (version 0.11.5)