FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4398901
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17450.026.603622
TATAACG6150.025.9305935
AGCGAAA18050.025.7427181
TATACTG25850.024.6766955
GTGATCG8100.024.1625989
CGAAAGC21400.022.0203253
TAGTGCG1700.003096653421.3209084
GCCGACC5450.021.28324145
TATACCG2755.224929E-621.0873575
GTCCTAT33100.021.056921
ACGTCCG8050.019.810339
TCTATAC39100.017.6145273
GTACCGT5953.0013325E-1017.0582793
TATAATA55750.016.9143262
ATACAGC46200.016.7884886
TAATACA55300.016.779134
GTATTAG20800.016.7544251
GTAATAT22700.016.631411
TCCTATG38900.016.595742
GTATAAG15250.016.1867871
Produced by FastQC (version 0.11.5)