FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4648147
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19500.036.0743832
AGCGAAA22950.030.6771871
GCGTAAG1902.0589505E-730.560671
CGAAAGC23650.029.4261653
CCGACGC5200.023.699242145
TATACTG29250.021.3117665
TCTATAC41650.019.6678523
GTACGCA5901.2732926E-1118.452951
GTCCTAT45700.017.7880521
GTATAAG15650.017.6236461
TAATACA65050.016.2692074
ATAATAC52400.016.186323
TATAATA49350.016.0177362
TATTCCG4551.377417E-615.9307196
GTATATA34100.015.7508451
ACTGTGC47050.015.560138
GTCTACG4201.1239552E-515.5531981
GTTCTAT54500.015.3153831
TATCGCT3406.281245E-414.9233375
TCCTATG48700.014.8913072
Produced by FastQC (version 0.11.5)