FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229496
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG701.6985508E-551.7736745
CCGTATA1157.149465E-637.821907145
CGTACGG900.003632686832.214735
AGCGAAA7000.032.1467441
GCGAAAG8200.029.18312
ATATACG1050.007716349327.6137494
TCTATAC7000.025.887893
TATCGAA1400.001000947225.8868377
TACGTCG1400.001000947225.8868375
GTTCTAT7700.025.4534341
GCCGACC2701.6512786E-724.163996145
CGAAAGC10650.023.1410983
TATATAC3854.292815E-1022.5930673
GACGAAC2603.2082298E-622.305227145
TTCTATA10150.022.1476172
TACGGGG2303.1484262E-522.0600877
GTTGTCG1650.0026047121.9645889
AAGCGGT1700.003097368421.3185716
TACACCG1750.00366413420.709475
TTACGGA2155.063589E-420.2286764
Produced by FastQC (version 0.11.5)