FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGTAGGAGTGGGAAGTGGACAGACAAATGTAGATGAACATGTGATGA14.545454545454546No Hit
GAGTTAGGCCTGTATCACTATAAACTAACATCATAGAACTCTAATTCTTA14.545454545454546No Hit
GGCACATAGGAGGGAACAGGTGTCAGCATTCAAATTGCTCCTCTAACACC14.545454545454546No Hit
ACTTAATATTATTGGCCAACTGCATTAAGGATCCCTGAAATATTAAACTT14.545454545454546No Hit
GATCATTCCTTACTCTTGTTTATCTGACAGATTTGGAGAATGATTGATAT14.545454545454546No Hit
ATCTTCCAATGCAAATGGTATAGTTGAACTCTTGTTCCTGTTAACGAAGC14.545454545454546No Hit
AGTTATATGTTTCTTTAATTGTATCCTTGTCTTTTCGTTTATACAGCTAG14.545454545454546No Hit
ATTACCAGTTTTCTTATAGTAAATGATTTCTAGTTCAAAACCACTGTGGT14.545454545454546No Hit
GGGTGAGTCTGTCCCCCCACAGTCAACAAGGTCTATGCCTAGAATCGCAG14.545454545454546No Hit
TCTCTCTACAGGTAATTAGTCTGTTCAGATGTCTTTTTCAATAGCATTCA14.545454545454546No Hit
GAATCGCTTCTATTTAAATTTAAAAATAGTTTTTAGAATTGAGTGAAATG14.545454545454546No Hit
GAGACACAGAGATCTGTCATGAAGATTCCTGGCTTGCAAGTCCAAGTCCA14.545454545454546No Hit
CAATTGTAGCTAAATGTTGACAATCTGAAAAGAAATTAGAAAATTAAGCC14.545454545454546No Hit
GTGAATGTGTGCCGTGTACTGATAGAAACTCTTCACACATAGTAGCTCAT14.545454545454546No Hit
ATCTTGGAGTAGGAAAAATCAATTTAAAAAGCATTAATAAAATACAAACA14.545454545454546No Hit
CTCAGCGGCCTCAGCCGTCCTGCAGGCCAATGCCCAACACCACAAACAAC14.545454545454546No Hit
GAAGAAAGTATATAGTGATACACGCCTAACTCAAGAAACAAGAAACATCT14.545454545454546No Hit
GAGGAAGAGGCTGGGTGGAAAGCAAGTTTCATAGGATAGGCTTAGGAAAA14.545454545454546No Hit
TGGCATGGAAGTCCCAGCAGATCTAAGACGTTGTCAGCCCACTCTGAATC14.545454545454546No Hit
ATTATAATAGTAACTATGATACTAACCCCTAAAAAAATGTTGAAAAAAAT14.545454545454546No Hit
GATCCAGAGGACCTACCGCCCCCCATTTTAGAGCGGCTGTTTCGCTCTGA14.545454545454546No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTACAGTTTACTTCTTG14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)