FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1790_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1790_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809822
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCG1252.7229362E-740.596878145
GCGAAAG16600.038.887562
CGAAAGC17150.038.4650573
TGATCCG8950.038.0669869
AGCGAAA18600.034.746321
GATGCGC7550.033.60378
TCTATAC22250.033.5580183
GAACGGT1601.8816754E-631.7123576
TATACTG22650.030.0811655
GCCCGTA1804.7093636E-628.190279
GGATGCG9700.027.6495577
GTAATAT11600.026.2919671
TATACGC1958.732028E-626.0370772
CATAGTG12900.025.8465824
TATACCA21150.021.9333325
GGACTGT22500.020.9402246
CTATACC19950.020.7093734
GTCCTAT12300.020.6630631
TAGTGAT18000.020.5375256
ATATACT19300.020.2801634
Produced by FastQC (version 0.11.5)