FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1789_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1789_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335229
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC30640.13120768883908174No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT23450.10041841720876198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG657.339713E-444.614014145
GTGATCG3500.039.3542489
ACTACGC1951.6916601E-1037.2309651
ATTCGAC1153.137818E-431.565381
TCGCCAT5900.029.49062145
CTACGCA2756.730261E-926.3803392
GCGAAAG7800.026.0616741
GTCTACG1701.02221304E-425.6236651
GTATAGG7450.025.3370441
GTTGACA13150.024.8442361
TCGACGA1801.521451E-424.165924145
GCACCGT16450.023.7966216
GTTACGT2252.6252888E-522.5867841
AGCGAAA9000.022.5867841
AGCACCG17400.022.0807655
ACGAACC5650.021.811625
TATACTG13300.021.8019755
GTACCGA3355.5135388E-821.6392757
CTATGCG5251.8189894E-1220.7132054
ATCGTCG4201.2714736E-920.7123188
Produced by FastQC (version 0.11.5)