FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1789_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1789_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305138
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2550.036.9744952
TATACTG25450.032.7534335
GCGAAAG16750.029.4437542
GTGATCG6900.028.3645959
ACTACGC5300.026.0247041
CGAAAGC20350.024.2246133
AGCGAAA19800.023.8317681
TGATCCG3052.0423613E-823.7663279
GTATAGG16050.023.0676421
GTATTAT31100.020.7748411
ATAGCGG5657.2759576E-1219.246653
GCGGTAT5305.820766E-1119.1469216
CTACGCA7600.018.1317252
GCCGACC7700.017.88814145
CGACGAA3703.4067307E-617.633684145
ATACTGT37050.017.6076376
CTATACG2901.8696825E-417.4969334
GTAATAT24950.017.1668071
TCGCCAT18450.016.89563145
TTAGAGT23250.016.835764
Produced by FastQC (version 0.11.5)