FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1789_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1789_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6559604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT148440.22629414824431476No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC146620.22351959051186623No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT82030.12505328065535662No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC77970.11886388263681771No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC72630.11072314731194138No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC69340.10570760064174606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC10700.042.735251
CTACGCA12150.038.2045482
AGCGAAA26200.032.9666751
GCGAAAG28000.031.6018982
GTTACGT6000.031.452241
TTACGTG6100.029.724982
CGAAAGC30600.028.9033243
TATACTG33900.028.6526535
CGCACTG18900.025.6964245
TACGCAC19200.024.5426523
TTAGGTA32900.024.016164
ATTAGGT36650.023.5386923
GCACCGT113200.022.283976
AGCACCG114150.021.8445075
GATTAGG38850.021.6560042
CACCGTG123500.021.071267
ACCGTGT120500.020.6936828
GGATTAG42300.020.5906641
CCGTGTT121550.020.515239
TAGCACC128250.020.4043524
Produced by FastQC (version 0.11.5)