FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1789_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1789_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916805
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT131430.3355541059613639No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC80660.2059331521482433No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC61190.15622426952579974No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT60350.15407966442036303No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT57980.14802881430145234No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC55520.14174818506410197No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC54400.1388887115901864No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA52790.1347782184714327No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA46740.11933195550965646No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC46710.11925536247017658No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG46050.11757031560161917No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC41790.1066941039954759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC8050.047.8035621
CTACGCA9000.042.7166632
TAATCGT2109.094947E-1237.970739
AAGGTCG9200.037.0327229
AGCGAAA15150.035.464951
TATACTG33400.035.3761985
TACGCAC11300.034.0043143
GTAGAAA28400.033.7147182
GCGAAAG17000.031.5752472
CGCACTG12450.031.440865
GCCGACC2901.2732926E-1129.99875145
GGTCGTT8300.029.6945257
CGAAAGC18200.029.4779223
AACCGGC2003.259629E-728.998795145
GTTGACA21650.027.5002211
GTCGTTT9050.027.2336528
TAGCATG28650.027.090882
AGCACCG79550.026.8813955
TTAGGTA17800.026.8795324
TTACCAC19000.026.709414145
Produced by FastQC (version 0.11.5)