FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1789_d06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1789_d06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23268
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT600.25786487880350695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.18910091112257177No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA270.11603919546157813No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC250.10744369950146124No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC240.1031459515214028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAC100.00700232145.306031
ATCTACA100.00700232145.306032
TATTGAA100.00700232145.306032
AGGGACA100.007047633144.993556
TATATCT300.001912302872.496774
AACACCT300.001912302872.496775
GTATATA350.00349345962.2740141
AATTATG350.003523478862.140097
GGCTGAT350.003523478862.140097
AGCTGTG400.00597816254.372585
ACTCCTT400.00597816254.37258145
Produced by FastQC (version 0.11.5)