FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912560
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG58390.11885859918250362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG17200.038.7718359
TATCGTC1301.65821E-533.45537
GCTATAT13350.033.173841
ATAGTGA24500.031.9532225
TATATAC17850.031.679283
CATAGTG24100.030.3803144
GTCATAG24100.029.7938882
TAGTGAT26650.029.3753836
GTGATCC30950.027.167798
CGTACGG2456.4608685E-826.6276865
GTATATA23450.025.6969971
AGTGATC32000.024.9172277
TATACCA42200.024.9064925
ATATACC23150.024.424834
TAGACCG2451.9177296E-623.6690545
CATAGCG4103.8198777E-1122.9867923
ATAGCGT4103.8198777E-1122.9851554
ACTCCGC12900.022.4764278
TCTATAC25450.022.2190633
TACACCG21100.021.986425
Produced by FastQC (version 0.11.5)