FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863279
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAAT335810.6905012029949341No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG325630.6695688238326446No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT301380.6197053469480159No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT243100.49986850435683416No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA230680.47433017928850063No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC213730.43947715111553337No Hit
GTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGCGTTG188370.3873312635363918No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG184890.38017559757521624No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA183060.37641270426804635No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA179200.368475672483524No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG173550.35685799642586824No Hit
GTCCTGTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTC171560.3527661069825523No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT158090.32506874477076064No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA155160.31904400302758695No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT154570.31783082977554855No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA138350.28447884647374744No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG124200.25538325068333523No Hit
CCCATATACAGCGCATCTGGATGCACCAGACGGCAGTCGCGCATGAAGAT123150.2532242135398771No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT122800.25250453449205773No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG121980.2508184292943094No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG120850.2484948940827783No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT117710.242038344910913No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA117190.24096910746843847No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG111000.22824106945128997No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT106890.2197899812040395No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG106560.21911142667323835No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG101320.20833680321445674No Hit
GGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT99910.20543752476467012No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG99120.2038131063424492No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA98670.20288780470953857No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGA96330.19807623621840328No Hit
GGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACAC93980.19324410546875884No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC93620.19250386416243032No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGC93270.19178418511461096No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT93140.19151687575399232No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCT92440.19007751765835354No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC89510.18405277591517985No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT89500.18403221365667072No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT89330.1836826552620156No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA89010.18302466298972359No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG88660.1823049839419042No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT87820.1805777542271377No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG86140.17712329479760466No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG85200.1751904424977469No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG84710.17418289183079974No Hit
GTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG84660.17408008053825413No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG83950.17262016018410622No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT83690.17208554146286897No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA82710.1700704401289747No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC81550.16768521814191617No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA80820.16618417327075005No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC80790.16612248649522265No Hit
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC80220.16495043776020255No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA79790.16406626064431015No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG79580.1636344532156185No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA79280.16301758546034475No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC78970.1623801554465619No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC78810.16205115931041586No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG78620.1616604763987425No Hit
CTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG77830.1600360579765216No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG76410.1571162172682258No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT76100.15647878725444292No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT75290.1548132443152038No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG74550.15329163718552855No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC73200.15051573228679663No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC71990.14802769900719248No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA71740.14751364254446434No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC71540.14710239737428182No Hit
ATCCTGATGTGTGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATGCG70780.1455396657275883No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT70580.14512842055740582No Hit
CCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGG67070.13791106782070287No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG66830.13741757361648385No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT66000.13571090616022646No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAC65670.13503235162942534No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC65450.13457998194222456No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT65160.13398367644545994No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT62270.12804118373632276No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT61940.12736262920552163No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGC60280.12394929429300684No Hit
CTTCAGGACCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCG60100.1235791736398426No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC59730.12281837007500494No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT59100.12152294778893005No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG58340.11996021614223654No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG57590.11841804675405214No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC57000.11720487350201376No Hit
CTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTT56970.1171431867264864No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT56550.11627957186910312No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG55890.11492246280750086No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA55380.11387378762353548No Hit
CTATAAGAAGGTGGGTTTTAAGGTTACGGGACGCTCTGAGGTGGACGATT54900.11288679921509746No Hit
AGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGC54410.11187924854815033No Hit
ATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGC53780.11058382626207544No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG53590.11019314335040206No Hit
CTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTG53390.10978189818021955No Hit
GGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACC53300.10959683785363743No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG53300.10959683785363743No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC53250.1094940265610918No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG52320.10758173651974315No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA52170.10727330264210629No Hit
ATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCTTCGCTT52080.10708824231552415No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT52060.10704711779850591No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGG51520.10593675583901316No Hit
CATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAACAACATA51500.10589563132199489No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC51420.10573113325392189No Hit
GGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATC51380.10564888421988539No Hit
GGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGC51080.10503201646461163No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT50690.10423008838275574No Hit
GTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGC50320.10346928481791812No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA49990.10279073028711698No Hit
GGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACG48990.10073450443620446No Hit
GCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGC48670.10007651216391246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTG57250.079.769724
TCATAGT57850.079.0717543
GTCATAG58450.077.932062
GGTCATA60650.075.5346761
CGAAAGC37450.066.976073
ATAGTGA68550.066.826915
TAGTGAT75050.060.9425246
TGATCCG78700.058.210599
GGACGGA24650.055.869976
AGTGATC83850.054.7200977
CGGTAGA11100.054.2878881
AGGACGG28150.051.240865
GTGATCC94050.049.0172588
TACGAGC5000.047.845303145
GACGGAT30150.045.678577
TAAGGAT18000.044.298934
GTTAAGG20050.044.135342
CGGTCCG1951.70985E-1037.175835145
TAAGACG6050.037.14324
TATGCTG56450.036.338275
Produced by FastQC (version 0.11.5)