FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990034
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18300.052.7113952
AGCGAAA19250.049.013161
CGAAAGC24950.038.646183
TATATCG1204.0638927E-430.203835
TATACTG28700.028.0358925
TCGCGCG1358.090986E-426.849731145
ACTACCG4500.025.7739355
CCCTATC10200.024.886962
TATACGC1600.002178245222.6528725
GAGTACG9600.021.8984369
GTGATCG6300.020.711829
GACGTAT6550.019.9210937
CTAGTAG12850.019.7465133
GTATATA39650.019.5857731
AACCGTA2608.093577E-519.5167127
TACACCG13550.019.2591235
TAGTACG21450.018.924926
GTATTAA43700.018.4349371
CCTATCC14850.018.0634543
GTCCTAT28400.017.8887441
Produced by FastQC (version 0.11.5)