FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547031
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC53980.11871482732358762No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG48790.1073007859414198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG22750.041.102865
AGCGAAA13800.039.977421
GCGAAAG15350.036.3794632
TCTATAC34700.029.0393753
CTATACC29800.028.7031464
GGATAGC24500.028.4033055
CAGTATG25900.027.9884159
TACAACG2603.7343852E-927.8798475
CCGACGC8400.027.61797145
CGAAAGC21350.027.503573
TATACCA35900.027.0566525
GTACTGA37600.026.6044946
TGGATAG27500.025.5683544
ACTGATC38750.025.4413688
TACTGAT37150.024.9758157
AGGTACT37500.024.7424354
CAGGTAC37950.024.4511993
GCAGGTA38150.022.8118572
TATCCCG5750.022.6917725
CGCTATA3203.3811375E-822.6633422
Produced by FastQC (version 0.11.5)