FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454290
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14690.10101149014295635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5550.050.9688532
CGAAAGC7300.038.7303053
CTATACG750.001484092238.6640973
AGCGAAA7350.038.521141
TATACGG850.00274513634.110695
GGTTCGC1358.0924993E-426.8472988
TTATACG1100.00968262426.358264
GTCCTAT12050.025.3037051
ATACACG1700.003097122821.3191836
ACATCGT1800.004313085720.134786
TATACCA15850.020.122085
CCGTTCA3259.852029E-720.073529
GACCGTA3259.855266E-720.0728287
CCTACGG1850.00503485219.6034052
GTACCGC1850.00505391419.59066
GTATATA6450.019.1342331
TCAGGCG6450.019.1052869
TCCTATG14450.018.5723612
TCTATAC14200.018.379063
TCGCCAT2801.427371E-418.123796145
Produced by FastQC (version 0.11.5)