FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1788_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1788_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533394
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC81860.1805711129454003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG5150.053.4851769
CGTACGG1502.6073394E-838.6594625
TAGACCG1901.2914825E-1038.150785
TCTATAC49600.031.5701793
CGAAAGC26850.031.3197773
GTTCTAT56300.028.49471
AGCGAAA30350.027.5054871
GTATAAG14850.027.374221
TTCTATA60200.027.2263492
GCGAAAG30900.026.9908662
CTATACC54850.026.960914
GCCGACC7650.023.691793145
TATTCCG4303.6379788E-1223.6002545
TATACCA66200.023.10375
TACCACT66500.021.9094497
ATACCAC70950.020.7396166
GAGTATA54600.019.518393145
CTAATAC19000.019.0774973
GTATAAC13000.018.4268381
GTATTGA15800.018.377361
Produced by FastQC (version 0.11.5)