FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1787eye_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787eye_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21107762
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG39750.040.667569
GCGAAAG73500.028.0254252
AGCGAAA77650.025.8034081
TATACTG130950.024.245755
GTATTAT246200.023.2352641
GCCGACC25600.023.220785145
GCACCGT108100.021.2569336
GTTGACA130850.020.3056451
CGAAAGC102600.019.9260033
AGCACCG116350.019.8742755
CCGACGC22450.018.083176145
TATAGAC64100.017.9912052
GTCCTAT205450.017.4554181
GTAATAT132800.017.383551
TCGCCAT60850.017.155561145
ACCGTGT136800.017.1156648
CACCGTG153550.016.9950227
CCGTGTT139450.016.6865629
GAGTGAT113550.016.597987
TTAGAGT140850.016.4178874
Produced by FastQC (version 0.11.5)