FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1787_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716200
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG10850.028.076472
AGCGAAA11300.027.6277221
CGAAAGC12250.024.8562853
GTTGACA17750.023.3147961
GTGATCG3004.567246E-721.7468479
GTATTAT26950.021.5519371
GTATATA14200.021.4741551
GCACCGT17050.021.2579156
GGTAGTG19300.021.0332187
CTATGCG6550.021.0278744
TAGTCGG2505.9860096E-520.2970568
TATACTG16100.020.2610385
TAGTGCA21100.019.926029
TAATACG2556.969883E-519.899444
GTCCTAT24450.019.5983811
AGCACCG18150.019.5701665
ATCGTGC3001.1080527E-519.330538
GTATAAG7350.018.7681451
TAGCACC24350.018.4576234
TAAGGTA23150.018.1618484
Produced by FastQC (version 0.11.5)