FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1787_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT130.17757137003141646No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC120.16391203387515366No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA120.16391203387515366No Hit
ACCTTATTTAGTTTGTAAACATAATTCAGAGTCAGTGTGGCTGTGTTTTA100.13659336156262805No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG90.12293402540636525No Hit
CTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTG80.10927468925010243No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC80.10927468925010243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAT100.006948329145.06
ACTGAAC151.1809738E-4145.08
GTTTTAC100.006948329145.01
ACCCTGA100.006948329145.01
ACCCCAC100.006948329145.01
TACTGCA100.006948329145.05
CAAAGCT100.006948329145.05
CGTGCTG100.006948329145.0145
TTCTACT201.9906856E-6145.04
TACCCAC100.006948329145.02
CCCCACA100.006948329145.02
GCATTCT100.006948329145.09
TCTACTG256.0414804E-6116.05
CATTCTA203.712498E-4108.752
TACTGAA203.712498E-4108.757
CTACTGA203.712498E-4108.756
ATTCTAC259.015249E-487.03
CTGAACA300.00185940872.59
ACATTCT350.003426369362.1428571
GGGGGGG300.001466925424.166666100-104
Produced by FastQC (version 0.11.5)