FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1787_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4446745
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT5600.036.3005521
TATACTG24450.035.8732265
GTTGACA25600.030.912191
GTAATAT20350.030.3247871
TAGCATG44350.029.6034262
AGCGAAA19150.027.6756171
GCGAAAG19700.027.247242
CGAAAGC21550.024.5571843
ACATGGT31150.024.4340325
GTATTAT55350.023.7412831
CGACGAA1550.001809840623.386263145
GTAGTAC4850.022.453951
GCATGTT58600.022.0189234
CTACTGA41700.021.5550756
TTACGTG9550.021.2672672
CCGTATA2053.6700696E-421.218756145
GCGATTA4451.1459633E-1021.17902145
AGCATGT60800.021.1043823
TAATATA32600.020.692952
TATTATC31350.020.5925182
Produced by FastQC (version 0.11.5)