FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1787_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349479
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT91700.14442129818840255No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC79890.12582134691681002No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT70070.11035551105846639No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG65110.1025438465108712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT7750.040.2740331
TATACTG35350.032.8087775
CCGACTA13100.032.6488234
GTCGGCT9800.031.0660536
GTAATAT28100.028.9314421
GTTGACA41600.028.6159971
TTACGTG11200.026.5508522
AGCGAAA27400.026.226661
CTACTGA60100.026.1726746
GCGAAAG28050.026.1156652
ACATGGT44750.025.7551175
ACCGTAC2559.393261E-825.6189141
TAGCATG60150.024.8395772
CGAAAGC29350.024.4531293
TAATCGT3902.0008883E-1124.1634399
ACGACTC5450.023.9406245
GGTAGTG51550.023.2017947
GGTGCGA4750.022.8913178
CAGGTCG24750.022.8447137
GTTATGT44150.022.688551
Produced by FastQC (version 0.11.5)