FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1787_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1787_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATACCAAAAATGAAACCAACTATTGGTGTGAAACCAACTAATGGT15.555555555555555No Hit
GTATCTTTGGGCTAAATACCCAATAGTGCAGATGATAGGTAGCTCTAATT15.555555555555555No Hit
AATCATGTGAGCATAATAAATTCCACAATACTGCCGAAAATGGTGACTGC15.555555555555555No Hit
GCCTCCCGACTGAGCACAGCGCTAGTTGAAAGACTTCTTCCCAAGCCCCA15.555555555555555No Hit
GGTTAACTCTGGTCAAACATGGCTTAAGAGGAAAAAAGAAGATAACCAGA15.555555555555555No Hit
ATTTTAAAGTTCACAAAGACAAAGCTCTGAGAAGATAAGTCACTTGCACA15.555555555555555No Hit
TGTCACCACATAGATAACATGGACAAACAGCCACTTCTGAGTCAAAGCAT15.555555555555555No Hit
CCTAAGAACCATCTCGATGGCTTCTAGAAGAAAACACAGGAAGATGTTTT15.555555555555555No Hit
CCAATAAACCCCTCCTGGAGGAACGGGCTATCAGATGCCCTCAGACTCCA15.555555555555555No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTACAGTCTCTTATACACAT15.555555555555555No Hit
TAACACTTGTGTGTGTGTTCACCAAACATTTTTATTGCTTAATTGAGTAG15.555555555555555No Hit
CAACAAATGAAGTATCACCATGCGCTCCACAATAAACCACAAATAAATCA15.555555555555555No Hit
TTTATCAAAATCGAAAAGACTCCAGCTTGAGTACCCTGGACACCACGGCC15.555555555555555No Hit
CATCCAGTCCCATTTGCCTTCTTAAAGGATCAAAAGGAAATGGAATTAAA15.555555555555555No Hit
TTTTGAAGCAGTATCACCTAATGATAAGCAGATTACTATTCTGAAAGTCA15.555555555555555No Hit
TCTAAAGAGATGCTACAATCTCTGGTTAGCCCTGCCCCCCACTCACATTT15.555555555555555No Hit
TTAGGAAGGAGTCCTCTATCCGACTGCATGGGTCACTTTATACAGACTGG15.555555555555555No Hit
GTTAATAGATTATTCCCTCCACCCCACATTCCTCATACAGTGAGAACTTG15.555555555555555No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)