FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40144250
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1141060.2842399596455283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGC186250.031.2975485
GCAGGTA171500.029.0695362
CAGGTAC175350.028.882183
GTATAGG147300.027.6357961
CGAAAGC124450.027.2464263
ACTGATC259050.026.0486458
TATAGCG36850.025.9567452
GACAGTA205200.024.6990057
TTGGACA227650.024.0470394
TATACTG245250.024.0044775
TATGGAT259700.023.2427142
GTATGGA322700.022.4635491
TGGATAG268900.021.8930994
GGACAGT282150.021.5838436
CTGATCC284650.019.5348179
GTCGGTA99050.018.094078145
CCTATAC161550.017.8947033
TCTATAC293000.017.7300573
ATAGGTG218050.017.4778583
CGCTATA40100.017.1669452
Produced by FastQC (version 0.11.5)