FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_pDZPB2_amp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_pDZPB2_amp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA160.4034291477559254No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT120.30257186081694404No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG120.30257186081694404No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG110.27735753908219873No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA100.2521432173474533No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC90.22692889561270801No Hit
AGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGG90.22692889561270801No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG90.22692889561270801No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT80.2017145738779627No Hit
CGTACGTACTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCA80.2017145738779627No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT80.2017145738779627No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC70.17650025214321735No Hit
GTTGTATGGGCCTCATATACAACAGGATGGGGGCTGTGACCACTGAAGTG70.17650025214321735No Hit
GTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATAGGCAA70.17650025214321735No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA70.17650025214321735No Hit
GACATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTATGGAG70.17650025214321735No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA70.17650025214321735No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA70.17650025214321735No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA70.17650025214321735No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG70.17650025214321735No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCCA70.17650025214321735No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT70.17650025214321735No Hit
CTTCAGGAATGTGGAGATTTCTAATGTTGTATAAATTTGGGCCTCCGTCG60.15128593040847202No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG60.15128593040847202No Hit
GTATTGATGTACACCCCCTTCATTATGTATTCTGTGGCTCTGCAGTGAGA60.15128593040847202No Hit
GAGTAAAGGGATTTTCATATAGGTATGGTAATGGTGTTTGGATAGGAAGG60.15128593040847202No Hit
GTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGAC60.15128593040847202No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT60.15128593040847202No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT60.15128593040847202No Hit
ATAATGACTGATGGCCCGAGTGATGGGCTGGCCTCGTACAAAATTTTCAA50.12607160867372666No Hit
TACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATA50.12607160867372666No Hit
TCCTCATAGTGAGAATTAGGTGCATTCAACTCTATTGATTTAGTAACCTT50.12607160867372666No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG50.12607160867372666No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT50.12607160867372666No Hit
ATTTTAAGGCATCCATCAGCAGGAATTTGGACCGCTGAGAACAGGGAGGC50.12607160867372666No Hit
ATATAGCGGTATTCCCTCTCCTTCATGACTTGGGTCCTCAATGCTTAATT50.12607160867372666No Hit
TTTCTGAACCATTCGGGCTGATTTCTTGTCATATATGTGATCATGGCCAA50.12607160867372666No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG50.12607160867372666No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT50.12607160867372666No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC50.12607160867372666No Hit
CCTTAAAATTAAGCATTGAGGACCCAAGTCATGAAGGAGAGGGAATACCG50.12607160867372666No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC50.12607160867372666No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC50.12607160867372666No Hit
TCTCCATTCCCCATTTCATTTTAATTTTTGAGGTTCCATTTGTTCTCACA50.12607160867372666No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA50.12607160867372666No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA50.12607160867372666No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT50.12607160867372666No Hit
GGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGA50.12607160867372666No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA50.12607160867372666No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT40.10085728693898136No Hit
ATATGATAAACAATGATCTTGGTCCAGCAACAGCTCAAATGGCCCTTCAG40.10085728693898136No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA40.10085728693898136No Hit
GGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACT40.10085728693898136No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT40.10085728693898136No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG40.10085728693898136No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG40.10085728693898136No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAA40.10085728693898136No Hit
CAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAG40.10085728693898136No Hit
GCAGAGGCCATGGAGGTTGCTAGTCAGGCTAGACAAATGGTGCAAGCGAT40.10085728693898136No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA40.10085728693898136No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACT40.10085728693898136No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC40.10085728693898136No Hit
ATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACTTGAACCGTTGCA40.10085728693898136No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA40.10085728693898136No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG40.10085728693898136No Hit
CCGCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGA40.10085728693898136No Hit
GTAGAAGGCCCTCCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTT40.10085728693898136No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA40.10085728693898136No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC40.10085728693898136No Hit
CTTCTATTGTGTTGGCCAATGCTGTTGCAGCAGGTTGGTTTCTATTTAGA40.10085728693898136No Hit
GTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCC40.10085728693898136No Hit
ACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGAACTTA40.10085728693898136No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG40.10085728693898136No Hit
ATTTTAATACAGCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTT40.10085728693898136No Hit
GAATTAGGTGCATTCAACTCTATTGATTTAGTAACCTTCCCCTTTTCGAT40.10085728693898136No Hit
CATATACCCTTTTCCCAGTCTCGCCATTTTGTTTGAGAACATTATTGGAG40.10085728693898136No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA40.10085728693898136No Hit
AAACCAGACTATTCACCATAAGACAAGAAATGGCCAGCAGAGGCCTCTGG40.10085728693898136No Hit
GTGATGTGTGTGTGCAGAGACAACTGGCATGGTTCGAACCGGCCATGGGT40.10085728693898136No Hit
AATCTACAGTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTA40.10085728693898136No Hit
GATTTGAAATAATCGAGGGAAGAGATCGCACAATGGCCTGGACAGTAGTA40.10085728693898136No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT40.10085728693898136No Hit
CACTATGAGGAATGTTCCTGTTACCCTGATACCGGCAAAGTGATGTGTGT40.10085728693898136No Hit
CTATGAGGAATGTTCCTGTTACCCTGATACCGGCAAAGTGATGTGTGTGT40.10085728693898136No Hit
TCCCTGTTCTCAGCGGTCCAAATTCCTGCTGATGGATGCCTTAAAATTAA40.10085728693898136No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA40.10085728693898136No Hit
GGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGGCCATGG40.10085728693898136No Hit
TAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGA40.10085728693898136No Hit
GATTACAGGTACACGTACCGATGCCATAGAGGTGACACACAAATACAAAC40.10085728693898136No Hit
ACTCTATTGATTTAGTAACCTTCCCCTTTTCGATCTTGAAAATTTTGTAC40.10085728693898136No Hit
TTACTATAATGACTGATGGCCCGAGTGATGGGCTGGCCTCGTACAAAATT40.10085728693898136No Hit
CCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTATTTAAAGCGACGGTAAAT40.10085728693898136No Hit
CTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTG40.10085728693898136No Hit
GCATAAGAATTGGTTCCAAAGGAGACGTTTTTGTCATAAGAGAGCCCTTT40.10085728693898136No Hit
TGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCAATCTACA40.10085728693898136No Hit
GCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAG40.10085728693898136No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC40.10085728693898136No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC40.10085728693898136No Hit
CCATAAGACAAGAAATGGCCAGCAGAGGCCTCTGGGATTCCTTTCGTCAG40.10085728693898136No Hit
ATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCAATTCA40.10085728693898136No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA40.10085728693898136No Hit
CATTTAAGAGAACAAGCGGATCATCAGTCAAGAGAGAGGAAGAGGTGCTT40.10085728693898136No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC40.10085728693898136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCCT100.00682755145.0145
ATTCCAT100.00682755145.08
Produced by FastQC (version 0.11.5)