Basic Statistics
Measure | Value |
---|---|
Filename | HHH35BGXF_n01_Chang_03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23946 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT | 63 | 0.26309195690303183 | No Hit |
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA | 53 | 0.2213313288231855 | No Hit |
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA | 47 | 0.1962749519752777 | No Hit |
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC | 43 | 0.17957070074333917 | No Hit |
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG | 35 | 0.1461621982794621 | No Hit |
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA | 32 | 0.13363400985550822 | No Hit |
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC | 32 | 0.13363400985550822 | No Hit |
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA | 31 | 0.12945794704752359 | No Hit |
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT | 31 | 0.12945794704752359 | No Hit |
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT | 28 | 0.1169297586235697 | No Hit |
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT | 27 | 0.11275369581558506 | No Hit |
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT | 26 | 0.10857763300760044 | No Hit |
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG | 26 | 0.10857763300760044 | No Hit |
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG | 25 | 0.1044015701996158 | No Hit |
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT | 25 | 0.1044015701996158 | No Hit |
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT | 25 | 0.1044015701996158 | No Hit |
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG | 24 | 0.10022550739163118 | No Hit |
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG | 24 | 0.10022550739163118 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTAACAA | 25 | 9.2742365E-4 | 87.0 | 4 |
AAGTACA | 35 | 3.3689525E-5 | 82.85714 | 6 |
GTACAGT | 35 | 3.3689525E-5 | 82.85714 | 8 |
GAAGTAC | 40 | 6.531119E-5 | 72.5 | 5 |
TAGTAAC | 30 | 0.0019126254 | 72.5 | 2 |
CCCTTAC | 30 | 0.0019126254 | 72.5 | 1 |
TAACAAG | 45 | 1.1702631E-4 | 64.44444 | 5 |
GTAAGAA | 45 | 1.1702631E-4 | 64.44444 | 1 |
CTTACTC | 35 | 0.0035240687 | 62.142857 | 3 |
TAAATAC | 40 | 0.005979157 | 54.375 | 4 |
GATGTAA | 40 | 0.005979157 | 54.375 | 2 |
AGTAACA | 40 | 0.005979157 | 54.375 | 3 |
AAGCACA | 40 | 0.005979157 | 54.375 | 5 |
TAAGAAG | 55 | 3.1557473E-4 | 52.727272 | 2 |
TCCCATG | 45 | 0.0095252935 | 48.333332 | 8 |
AGTACAG | 60 | 4.8481883E-4 | 48.333332 | 7 |
CTGCAAA | 45 | 0.0095252935 | 48.333332 | 145 |
TTACTCC | 85 | 5.1860494E-5 | 42.647057 | 4 |
GTAAATA | 85 | 5.1860494E-5 | 42.647057 | 3 |
AACAAGA | 80 | 0.0019971686 | 36.25 | 6 |