FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_Chang_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_Chang_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23946
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT630.26309195690303183No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA530.2213313288231855No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA470.1962749519752777No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC430.17957070074333917No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG350.1461621982794621No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA320.13363400985550822No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC320.13363400985550822No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA310.12945794704752359No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT310.12945794704752359No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT280.1169297586235697No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT270.11275369581558506No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT260.10857763300760044No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG260.10857763300760044No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG250.1044015701996158No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT250.1044015701996158No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT250.1044015701996158No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG240.10022550739163118No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG240.10022550739163118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA259.2742365E-487.04
AAGTACA353.3689525E-582.857146
GTACAGT353.3689525E-582.857148
GAAGTAC406.531119E-572.55
TAGTAAC300.001912625472.52
CCCTTAC300.001912625472.51
TAACAAG451.1702631E-464.444445
GTAAGAA451.1702631E-464.444441
CTTACTC350.003524068762.1428573
TAAATAC400.00597915754.3754
GATGTAA400.00597915754.3752
AGTAACA400.00597915754.3753
AAGCACA400.00597915754.3755
TAAGAAG553.1557473E-452.7272722
TCCCATG450.009525293548.3333328
AGTACAG604.8481883E-448.3333327
CTGCAAA450.009525293548.333332145
TTACTCC855.1860494E-542.6470574
GTAAATA855.1860494E-542.6470573
AACAAGA800.001997168636.256