FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_Chang_02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_Chang_02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4736472
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT183770.38798920377867746No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA128020.27028556275641447No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT102960.2173769843883802No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG88670.18720684931738224No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG86610.1828576206087569No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG82420.17401137386645588No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG80870.17073889595462616No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA79850.16858539436103495No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA79660.1681842519073268No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA74560.1574167439393709No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC73480.15513656578145085No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT73070.15427094259187007No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT72420.15289861314497374No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA71560.15108291572292626No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA69910.14759931020388173No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT67860.14327119425597787No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT67790.14312340493092748No Hit
TTTGAGAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC67150.14177218824475263No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT67010.1414766095946519No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC65520.13833080824715105No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA65010.13725405745035546No Hit
TGCTAATACTGCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTT64180.13550169831047243No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT64060.13524834518181464No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG62620.1322081076379212No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA62250.13142693549122636No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA62220.13136359720906193No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG61260.12933677217979966No Hit
TAGTTAGGACTTCTGTTTGTGGATTATAGGAAAATAGAGGGGACCCTTTC60860.12849226175094036No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT60290.1272888343898159No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT60220.1271410450647655No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG60140.12697214297899365No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA59730.1261065197894129No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT58940.1244386116924158No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA58220.12291849292046908No Hit
AGTAAGATGTTTGCCTTTTCCCCCGGTTTCAGCTTTTCAAGTTCTTCAAT58060.12258068874892536No Hit
TGACTACCCTGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT57910.12226399733810314No Hit
TTTGAAAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC57190.12074387856615641No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT55010.1161412967288733No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG54860.11582460531805107No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA54820.11574015427516515No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG53290.11250990188477838No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA52660.11117979795932499No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA52140.11008193440180793No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA51820.1094063260587205No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT50660.10695724581502856No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA49790.10512043563225962No Hit
ATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGC49210.10389589551041366No Hit
TATGAAAACTGACCAGTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCAC49020.1034947530567055No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT48900.10324139992804772No Hit
AGTTAGGACTTCTGTTTGTGGATTATAGGAAAATAGAGGGGACCCTTTCC48050.10144681526672174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA50950.0121.110244
GCACGCA11250.0119.882532
GAAACAC25650.0119.294534
AGCACGC11500.0117.2751541
CACGCAC11600.0116.8904653
AAACACG27100.0112.911615
AACACGT6400.0103.099596
TAGAAAC31450.098.677612
TAGTAAC63450.097.936532
CGCACTT15000.095.712685
ACACGTG7000.094.2495357
TAACAAG69350.089.290845
AACACGA27450.089.283226
GCGCAGG259.389504E-486.99866145
ACGCACT16150.086.6523364
GCCCGCA608.2945917E-1084.594532
ACACGAG29650.082.6471567
AGTAACA78750.079.369243
CTGTCCG755.638867E-1177.33214145
ACGTGCA12850.075.048842
Produced by FastQC (version 0.11.5)