FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_Chang_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_Chang_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12827
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT290.22608560068605285No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT280.21828954548998208No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA260.20269743509784047No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG220.17151321431355734No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT210.16371715911748655No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA200.15592110392141575No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT200.15592110392141575No Hit
GGGTCATACTTGCCACTAACGAGAAAGCCTGCTAATACTGCATTACCCAT190.148125048725345No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA190.148125048725345No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA180.1403289935292742No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA170.1325329383332034No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG170.1325329383332034No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG170.1325329383332034No Hit
TTTGAGAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC150.11694082794106181No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA140.10914477274499104No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG140.10914477274499104No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC140.10914477274499104No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT140.10914477274499104No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG140.10914477274499104No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG140.10914477274499104No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT130.10134871754892023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAT100.0070098704145.0145
GTAACAA151.2020496E-4145.04
TAGTAAC203.778508E-4108.752
AGTAACA203.778508E-4108.753
TAACAAG259.174954E-487.05
ACAAGAG400.005915851354.3757
GGATGCA450.00942481448.333332145
TAACATA450.00942481448.3333329
TGATAAC750.001291350329.06
GAACAAC950.007581659610.68421145-49
Produced by FastQC (version 0.11.5)