FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_Chang_01_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_Chang_01_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746570
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT93890.2506025511334367No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG88760.23691002703806413No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA86370.2305308588922668No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT78830.210405784490881No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT77460.20674910651609338No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA66660.17792273999951955No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT60290.1609205219707626No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA60130.16049346468903555No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG59030.1575574458771623No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG58250.15547554162874308No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC57000.15213915661525074No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA54400.1451994757871867No Hit
ATATAGCGGTATTCCCTCTCCTTCATGACTTGGGTCCTCAATGCTTAATT51400.13719215175480506No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC50120.1337756935009889No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT47530.1268627037530328No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG47160.12587513378903903No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG46740.1247541084245056No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA46540.12422028682234684No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA45270.12083051964863863No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT45080.12032338912658778No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA44640.11914898160183848No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA44150.11784111867654949No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG42900.11450473366305715No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA42760.114131058541546No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCCA41430.11058114488719015No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT40230.1073782152742375No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA40020.10681770259197078No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT40010.10679101151186285No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT39460.10532300210592623No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG39230.10470910726344362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC351.904482E-9124.282425145
CCTAGCG458.54925E-11112.7748145
ACGGCCG1200.0108.74713145
AGCGAAA3050.090.341151
ATTAGTA1900.076.327021
GGTACTG11150.076.08645
GCTCACG502.307841E-672.498085145
TACTGAT11500.071.8705447
TTAGTAG2100.069.057772
GTACTGA14050.063.995186
GGATAGT350.003565190462.1521
AACAGAC9300.060.8072857
CTATTCG5350.058.2718249
TAAACTA5600.058.266725
TAGATAT8700.056.6750033
AGGGGTT3850.056.501065
ACAACCG805.8791557E-754.373566145
ACAGACT10500.053.8564348
TAGTAGA2700.053.71163
AGATATT9350.053.5098344
Produced by FastQC (version 0.11.5)