FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_Chang_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_Chang_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAATCCGAACTGAGACCGGCTTTTTGGGATTAGCTCCACCTCACAGT10.9523809523809524No Hit
GTAGAAGCAAGGGACAACATATGCAGACGCAGTAGTGGATTGTCCTCCGT10.9523809523809524No Hit
GTTGAGGATATTTGAACTAACCGAAGAGATGCTATCTGCACGATTATCAT10.9523809523809524No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10.9523809523809524No Hit
GGAGAAATTATAGAAGGGCTGCATGAGCTGAGGCTGGAAAAACTCTGTCA10.9523809523809524No Hit
GATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCC10.9523809523809524No Hit
GCCTTAAAGTACGACGAAAATTGTGGGAAACAGATCACAATCTGTTGGTC10.9523809523809524No Hit
GCGATTACTAGCGACTCCGCCTTCATGGGGTAGAGTTGCAGCCCCCAATC10.9523809523809524No Hit
CACTGTAGCTGTGACATTAGAAATAGAAATAGAAATAGACACATAGAAAA10.9523809523809524No Hit
CTCATGGAAACCCTCTTAACAGACTGAAGTTTCCCTCCTAAGAACTGTGG10.9523809523809524No Hit
CATCTGTAGTTTTGTTTTCTGTTGGTGATTTCATGGTTTTGAATGGAGTG10.9523809523809524No Hit
TATGTTACCCATCAAGTAGAACCACACTCACTTCTTGAGTAGTCTCTTGT10.9523809523809524No Hit
CCATCCAAGAGAGTTGGTTTCATTGCTATACCAAAGGATACAGATATAAT10.9523809523809524No Hit
GGCTGGTTATTTTAGTCCCCTATGAACCTGAGTGTTGCTCTATGATGGGA10.9523809523809524No Hit
GTTTAGGTTACATTCCTAGAACACAAGAACTTCCGTGATCACATCCCACA10.9523809523809524No Hit
ATCAAAACTTGACCTTTGTATGTTGCTTTGGTTAAGTGTTGATGTTTTTC10.9523809523809524No Hit
GCCCAGTGACAAAGCTATGCTTCCCAACATCCCTAAAGCCATGGCCCAGT10.9523809523809524No Hit
GAAACAGAAATATGAACAGACCAATGAGCAGTAGAGAGATTAAATAAGAA10.9523809523809524No Hit
GAACTTATCTGGAATTACTTAAACACAGAGAGTAAACAACATTCTACTGA10.9523809523809524No Hit
GCGCGTTCTCTGTGATTGTTTGATAATTATATACTGTCGATGGCATGATA10.9523809523809524No Hit
CATAGATTCAGTTCCAAATGTTTTAATTGCAGCACTAACTATTTGGCCAA10.9523809523809524No Hit
GATGAGGAGCACCTATAATTTAAATGAACAAATTTAAACTTTTGAGTTCA10.9523809523809524No Hit
CGCGTTCAGAGGTGGTTGTGGTCTATGCCACGTTCGTGTTGGTTTGGGTG10.9523809523809524No Hit
TCACTGAGGGGTGCAGTGTGTGTGATCAGATAGTAGGAGCTGGTGGAGCT10.9523809523809524No Hit
GGGCACTGTGTGGGGAGAGGAGATTTGGTAATCCTGATTGCTCTGCCGCC10.9523809523809524No Hit
GGCCTGGCCCTGGTTCTTCCAAGCATTGTCCCCTTCACTAGCGAACGAGA10.9523809523809524No Hit
CCTCAGGATATTGTGGGCCAGGTTGAGCTGCATGAGGTTCTTGAGGCCCT10.9523809523809524No Hit
ACTCCATCTTATTGTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTT10.9523809523809524No Hit
CAAGAAGAGCATTTGATGCCTAAATTAATAGATGAAGGCAAACTTAAATA10.9523809523809524No Hit
ATCCTACTAACAATTCACAGGAGTAAATTGATAGTCGTGTTACATGAAAT10.9523809523809524No Hit
TTGTTAATGGTAGGGCACACCTTGAGTAACTGTTCTAGTATACGCTCGCG10.9523809523809524No Hit
CACCCAGGGGTAGGCCCCCGGCGTCCGGTGTGACCACCTTTCGTGCCTGG10.9523809523809524No Hit
GGGTGATGAAAAATTTGGTGACACTGAAGGCTCAGTTTCTTCTAGGGAAA10.9523809523809524No Hit
GATGTCTACTCTTGGAGCATACATGTTGTTCGATCCATAACACAATGTCC10.9523809523809524No Hit
ACAATGGGTTGCGATACTGTGAGGTGGAGCTAATCCCAAAAAGCCGGTCT10.9523809523809524No Hit
ACTCACACAGCATTGAGACTCAGTTCTTGGCCTGTGGTGGGGTGGAGCAA10.9523809523809524No Hit
ATACTATACTGTCAAAACAAATGTAAACCCAGATAATATACATCAAACCA10.9523809523809524No Hit
CCTTGAGTTGTTGGAATTTTCACCGCCTCCTCGCAGTCCTGACCTACTGG10.9523809523809524No Hit
GGGTTATGGAAATGCCTGTTGTCAGGAGAAAATTAGGTTACTTAAAGATA10.9523809523809524No Hit
TCTTAGAACTATTATTATTTTAAATTAAATTCTCTCAAACTCGTCGTGGT10.9523809523809524No Hit
CTGTCATTACATGCATATTTGCATGACAACAGACCATATTTCAATATTTA10.9523809523809524No Hit
GAAGTACGGTGGGGCTTCGCCTGGGAAAAGAGGCCCAGTGGCTCCCACCC10.9523809523809524No Hit
GTGGGAACCATGGGAGCAGTTGCCCATAGTTTTCTCAGGTCGGGTAGCAT10.9523809523809524No Hit
CCCGCGGCGCCATGGCCCAGTGGCCCCCATCGAGGGCCACGAGGGACGTC10.9523809523809524No Hit
TGCCTGGCCCGTCCACAAACGAACATTTTTTGCACTGTGTGCCAACAGTG10.9523809523809524No Hit
GCTCTAATTATGTGTTTAGGGGGTTTTCCATGTACATAACAAACCTTAAC10.9523809523809524No Hit
TACTAATCTGTCTCCGGAGTTGAATGCAACTAGATGTGATTCGTTCCATT10.9523809523809524No Hit
CCCGCCACGAGCTGACGCCAACCATGCCCCACCTGTGACCCCGCCCCCAA10.9523809523809524No Hit
GCTCTGGGCTGTTCTGTTCTTTGAGCCTTATAGGAAATTCGATGTAGTTC10.9523809523809524No Hit
TCTGGTCACGGGATCCTGGGGGGCTCTCATGACGACGTGGGAGGAAGGCT10.9523809523809524No Hit
GTATTGTACTGTACTGACTTTTTCCTTTCTTCTCTACATGGACAGAACCC10.9523809523809524No Hit
CAAGGAGGCAGAGAGGCAGGCAGGGAAGCAGGCTCCTGGCTGAGCAGAGA10.9523809523809524No Hit
TACTTGAGTAATATATAGTATGGAATCAATTTGCGCCACCGTCTGAGAGA10.9523809523809524No Hit
CCTCAAATGTTTCACTGTTCATAGTCAGGCTAGGAGAACAATTCCAATCT10.9523809523809524No Hit
CACTATCGCAACACAAACTAACCGCCAAAGAAGCAATCGTCAAGCCCAAC10.9523809523809524No Hit
GATTGTCATCTTAGTTAACATTTATAACTGTCTCGTGATGTAGGTTTTAA10.9523809523809524No Hit
ATGTACTGCCAATTGTGACTTTCTAATTAATTTGGCCTTCATTGAACTTC10.9523809523809524No Hit
TACCCAGCCAGGTTGGAGCAACTAAAAATGTAGAGCATATATGACGAAAC10.9523809523809524No Hit
CATAGATTCAGTTCCAAATGTTTAAATAGCAGCACTAACTTTTTGGCCAA10.9523809523809524No Hit
CAGTCTCCCATGAGTCCCCACCATTACGTGCTGGCAACATGGAACGAGGG10.9523809523809524No Hit
GAGCAGTATCACGGAAAATGAAACTTCACTCACTGAACCTTCGCTGAGGA10.9523809523809524No Hit
CCTGGGAACCCGCCCCCAAGGGGCAACCGTCTCGCTACGGCGATCGAGAA10.9523809523809524No Hit
CGGGTATTCAATGGCAAAAACCTTGGCTCAAGTCTCAACATTATGATCAT10.9523809523809524No Hit
GTCGCATGCGTCACCCTGGGTGTACACGCTGGACTCATGGGACTGAGTGA10.9523809523809524No Hit
CTCCTATTCAACCTAGTAAGTTGCGTGATAAATACATTCAGTTTCATGAA10.9523809523809524No Hit
CATATAACCTACTAAGATTGAATTCTAAAGAAACAGAATTCTGACTGTTC10.9523809523809524No Hit
GGGAGAAGCCGGCTCCCTGCTGAGCAAGGAGCCCGCTGCGGGCCTTGAAG10.9523809523809524No Hit
GAGTGGGATAGGAACACTACAGAGATGACGGCCCTGTTGGTCTAGGCCGA10.9523809523809524No Hit
TAGTGATTCTGAGACTACGCACCGCTTTAGATCAATAAGACCTCTGAGGG10.9523809523809524No Hit
GGGCGGGGCCCACGGCCGGGGACCAGGTTCCCCGTTTCGGCGGCGCCATT10.9523809523809524No Hit
CTCTCGGCTAGCATTACCCACCTGAAGACAATAAACAGGCACATGCTTCA10.9523809523809524No Hit
CGTAAAAGCCCTCTAAGTGATACAGGCGTGTCAGATTAGGCTGCCTCCCT10.9523809523809524No Hit
CGTCACTCCTTGACCCTTTCCCGTAAAATGCTAGGCGTCCATCTCGTGGG10.9523809523809524No Hit
ATTATTCTATCAGTGTCTAATAATTCAATGGCAACAATGAAGAAATGATC10.9523809523809524No Hit
TTACTGCCCCAGCATGGAGCAACTAAATTTGTAGAGCATATATGAACAAA10.9523809523809524No Hit
TATATACACACACACACACACATAAACTCACACACACACATATAGATACA10.9523809523809524No Hit
CTCGGGCACTGTGATGAGTGCCCTATGTGCTGCCGTCTTAGGTCTCTCCT10.9523809523809524No Hit
TAACAGTCTCCCTCACCAGGTTACTGTGGGCTTCAGAAGGGAGTGTGAAT10.9523809523809524No Hit
TCTAGCAGCAGTGGAGTCTACTGGGAAGGCACGAGGCCCTTTCACAGGGC10.9523809523809524No Hit
AGTAGAAACAATGTAGTTTTTTAATCAAGCTCTATTTTGACAAAATGACC10.9523809523809524No Hit
CTCAGAAACATGGAGCACAATGGACAGAGGCAAAGGCCATCCCGCACCAC10.9523809523809524No Hit
TCCCGGTTCTCCGCGTGCAACCGTCAGGAGTAGGGCTGCCGTCCACGGAC10.9523809523809524No Hit
GTAGTACATCATTAGTTTTTGATGTAGTGTTTCAGGATTCACTGTTTGCA10.9523809523809524No Hit
GGGGCCCTGGGCCGCCCTCAGGGGGCTGCAGCGATGGGTGTGAGTTGGCA10.9523809523809524No Hit
GGGTAGGGGATGCCTTCACTAAAAAACACATTTTTATTGAAGAATAGAAG10.9523809523809524No Hit
GTATAGATTATAGAGTCGTAGACTACTGTAAATATTCCATTTTTCAATTA10.9523809523809524No Hit
GGGTCATACTATTGCTATCCAAAATAGACCAGTTTCACAGTCTATCCAGT10.9523809523809524No Hit
CAGTCTGAGGCTGCTTCCCTCACGCAGCAGGGCGGCACCTCCAGGGGGCG10.9523809523809524No Hit
GAGACAGACAGACTCACACTGAAAGAATCGACGCAGTAGTGTCATTGAGT10.9523809523809524No Hit
CAGTTCGGAGAGGGAGAAACTGTGGGCTTTGAAGTTGGGCTCCCCTGTGC10.9523809523809524No Hit
GCCCCAGGACAAGGAGGTTGGTTTGGGCTGAGCTCTGCGCAGGTCAGGTA10.9523809523809524No Hit
CATGTGGAGAGTGTCTTTTCTGCCCGTGAAAAGAATGTGGCTTCTGCAGA10.9523809523809524No Hit
GCCATCCCAGCGACAGAGACCCGAAATCCAGTCACCCACACCCTAAGAAA10.9523809523809524No Hit
ACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGCGGATTA10.9523809523809524No Hit
GGTCTGTTAGGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGG10.9523809523809524No Hit
GGTGTTGGCGGGGGAGTCGGGAGGGTGTGTGAGCATGGCTGATCGACGTT10.9523809523809524No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCATGAAGGCA10.9523809523809524No Hit
AGCTTTGCCATATTCCGACGATTAACGCAGACTCTAAATTTGTTTCATTT10.9523809523809524No Hit
GCAAACTCAAGCACAAATTCTCTGTGAAGAAAAAACTTCCTCGGTGCATT10.9523809523809524No Hit
GGGTGAGCAGGAGGCGGAGAGAGGGCAGGTTCTCGCAGGGTCCGGGGGGA10.9523809523809524No Hit
CTGTAAGAGAGCTAGGTCTTTTGGGGGAGAAATGTTTGGGTGTCATATTT10.9523809523809524No Hit
CGCCTGTTCCCAGTATTGTACTGGGATAGCCCTCACGGGAGAATCGATTC10.9523809523809524No Hit
TCTCAAGACCCTGAGATCATGACCTGAGCTGAAAACAACAGTAAATCACT10.9523809523809524No Hit
ATTCAAAAGAGAACACATAACAACCAAGTGACCTATATTCAAGAATGTAA10.9523809523809524No Hit
GTACTTAGATAAAGGCTGCTTATGTTACATTAGAATATTAAAAGAATTAG10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)