FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348034
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC46490.1069218869953639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15700.039.7161452
AGCGAAA16650.037.015021
CGAAAGC20800.029.6294693
GTGATCG3900.027.8849569
ATAGTCG1751.2560765E-424.8588755
TATAATA28100.024.7704092
TCGACGG3702.6011548E-1023.51326145
TATACTG21600.023.497015
ACGCGCT1902.1946282E-422.8950169
TAATACA56900.020.770344
GTCCTAT28250.020.0193231
GCACCGT18750.019.7213756
TATTATC27550.019.4750422
AACGCGC2256.873241E-419.3331248
ATAATAC44350.018.9641193
GTATTAT38100.018.8401131
ACCGTGC5801.0913936E-1118.7497988
ACGATTG3502.0024181E-618.6426568
AGCACCG19600.018.4961875
TTATAAT25700.018.3380641
Produced by FastQC (version 0.11.5)