FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408846
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA3600.036.249653145
GTCGATA900.00362935832.2223859
AGCGAAA15200.029.1027981
TATACCG2001.0643485E-525.3814585
CGAAAGC19100.023.9196993
GCGAAAG18900.023.7891222
CGTTAAC3402.5102054E-923.455656145
TAGACCG2202.2264592E-523.0740535
GTATTAT22500.021.5943831
CCATCGA5850.021.0684839
GTGTACA18350.020.5501881
GTACACC15700.019.399843
TATACTG20250.019.3382555
GTATATA15800.018.8180771
ATATAGG7750.018.714441
GAATAAC17850.018.6882291
CTATACG1950.006833817318.5944734
TATAGCG1950.006833817318.5944734
TGTACAC17000.018.3429012
AGCGTAC2000.00790827818.1296123
Produced by FastQC (version 0.11.5)