FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646441
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC33100.029.1366653
GCGAAAG32950.028.8291662
AGCGAAA34750.026.7098451
CCTACGG8100.025.066243
TATACTG26750.022.4997795
GATGCGC8750.021.5431128
CCCTACG10000.020.3036562
GTAATAT26650.019.8628641
CGAATAC4752.746674E-1019.841637145
GTCCTAT51800.018.3382421
GCACCGT33350.018.0472076
TAAGGTA31350.017.3477754
ATCGATA5902.6557245E-1017.2035928
TAGTACT15900.016.8742564
ATACCGC8600.016.8639496
GTATATA27600.016.8146231
AGCACCG35050.016.551065
CTACGGT12800.015.86237054
TAATACT31800.015.7341044
TACACCG12450.015.7260025
Produced by FastQC (version 0.11.5)