FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426493
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG86220.15888714866120715No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC81640.15044707511831307No Hit
ATCCTGATGTGTGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATGCG72070.1328113755974623No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGA61660.11362771499014188No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG60000.11056864903354709No Hit
CTATAAGAAGGTGGGTTTTAAGGTTACGGGACGCTCTGAGGTGGACGATT59590.10981309659848451No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGC56060.10330797441367749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG12100.037.1488469
CGGTAGA6000.031.422821
GATGCGC52250.026.9182248
TATACTG22100.025.9213665
GTTAAGG28200.025.7142542
TACGGGC7850.024.012352145
TATGCTG63650.023.2410015
CGAAAGC29650.023.2338923
TTGTACG16500.022.8529594
CATATGG67150.022.1376172
GTATTAG12800.022.094171
TATGGAT66150.021.814554
GCATCGA33300.021.553707145
CATAGTG21650.021.4360684
GTCATAG21150.021.2571162
GGATGCG69450.021.1917487
TAATACG3106.242717E-721.0525074
CATATAC40400.021.00043
TGTACGT12800.020.9611365
CCATATG69000.020.8084222
Produced by FastQC (version 0.11.5)