FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAGGAAACATGCCCTGAGAAAGAGACCAAAAGAGCTGTTGTGGCAA12.1739130434782608No Hit
AAAATGACCAAAATACAAAATAGGAAATCACATAAAAACTAACACAAAGC12.1739130434782608No Hit
CTAGTAGTAAGATATTTTTGATTTAGGAACACTAGTTCATAGTTATTTAC12.1739130434782608No Hit
AGTTAAGCCAGTATGTCCACTTTACTCCAGAGAACTCTGACACGCAAACA12.1739130434782608No Hit
ACCCAGAGCCCAAGATCAAAGCTGAACGGAGACCCTTCACAGAGACCGAT12.1739130434782608No Hit
TCTCTGATGTCTACCCCCAGTTCTCCCAGTGCCATCGTTGCTACAGCCCT12.1739130434782608No Hit
GTAAACACTCAGAAAGAAGGGAATTTCAGGTTTACAATAAAAAGGGATAT12.1739130434782608No Hit
GGCTTAAATATGTTGTATAAAGGAAAAAAAACTTCGCCTCCCACGGTGCC12.1739130434782608No Hit
ATCTGTCAGCCTGTTGTCTTCAGGGACCTGCCATTTAGGAAAACAGGTGA12.1739130434782608No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGAAGTATATTCCATTAGCT12.1739130434782608No Hit
ATATGATAGAGGATAAAACATACACATTAAACAATGGTTCACGAGGATCT12.1739130434782608No Hit
GCTGTCGATCACCAGGCAACCTCACTAGGTCGATTCTTCAACCCTGGAAG12.1739130434782608No Hit
CTATAACACATCAAATGAAAACTGCAACAGAAAAAAATAAACAGCAGAAT12.1739130434782608No Hit
GAGCATAACAGTGCAGGGCCGCGGTGTTCCCGTATCAAAGAACAAAGTTG12.1739130434782608No Hit
GTGGGGTCTTGGGAAACACGTTTGGGAATGATTATGACATAACATATGTG12.1739130434782608No Hit
ACAATGTTCCAGCCTCATTGGCAGGTAGGGAGAAATGAGGTTTGTTATAC12.1739130434782608No Hit
CCCTAAATCCATTGTCCTTCATTGTAGAATTGTGAAAGCAGGTTAGTAGA12.1739130434782608No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGCCACAACAACAA12.1739130434782608No Hit
AAACAAAAAAAATACCAGTATATAGAATAAATAAGAAATACATATCAACC12.1739130434782608No Hit
CTTAGGAGGTTGTCTTAGCCATGAAAGTCACTAAAACTGGGGATTAAAAT12.1739130434782608No Hit
TCGTAAGATTGCTCAGTTCAAAGATCGTAAATGCTGCTCCATATCTTTTG12.1739130434782608No Hit
CATTCAGATAGACACACAATGATATTACCTAAGTGATAAACAATATGTCA12.1739130434782608No Hit
GTCTTGGAATGCTGTCTTTGCATCTGCGATATAATTACCTCCTCCCGTCT12.1739130434782608No Hit
TGCCCAGTGTGGGGTTGAAACTCAAAACCACAAGAACAGGATCACAGAGC12.1739130434782608No Hit
ACACTATAGATGGTGAGGGGCTTTTCCTAGCTCCTGGATATCCTGATATC12.1739130434782608No Hit
ACACTATAACTTATGCATGGATGATTTTCTACCACATGTGTTTTCTTTGT12.1739130434782608No Hit
TAAAAGAAACATTAACTAAGTACAAAAAACCATACAACAATACATATTCA12.1739130434782608No Hit
CCTATAATTTTCCCAAATGAGGACTGAAATCAAATCAACCATCTTTTAAA12.1739130434782608No Hit
GTCGATTCCTCTGCTCCTGAAATGCTAATCCCCAGTTTGTCACATAAATC12.1739130434782608No Hit
CGGTAGGCCCTCCGAGGACACTGGCCGAGGCCATCAGCAACTCCAGACCT12.1739130434782608No Hit
CAGTCTGACATTGCACTACACAAATACCACCTTTAAAATCAGTCTTCAGC12.1739130434782608No Hit
GAAATTAAGATAAAATGAGGTCATTGGGTGGGCCCTAATCCAATGAGATT12.1739130434782608No Hit
GCACAATGGCTGGATTTTAAAAGCATGCAAAGCAATGATATATGATAAAC12.1739130434782608No Hit
CATGTATGTTTTAATAGATTACAGGTAGTTTTGTATCATGATCTTGACTT12.1739130434782608No Hit
CTCAACAATGGCACAGAATGTGTTTACATTAGCTACATGGAAGAATTTCC12.1739130434782608No Hit
CACATAGGCAAAATAATTGTGTTACTAAATGAATCAATAAATCCAAAATA12.1739130434782608No Hit
GGTATAACTAATTGACATATACAAAAAGACCCATCCATCATAAATAATCT12.1739130434782608No Hit
GGCTTTGGTGTATACGTTTTCAGAGCACACAGACATACAAACATTCAGTC12.1739130434782608No Hit
AAATGGTATAGTTCAACTCTACTTCCTCTTTACTAAACACTGGGACAAAA12.1739130434782608No Hit
GGCACACAGTGGGCGCCGTGTACGTGTCCACCGTCATAAACACTCAGGCC12.1739130434782608No Hit
ATTTTATAATGACAACTAGATCTTTACTTTTAGACAAAAGCGTCTATTTA12.1739130434782608No Hit
GATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGATTTTCCCTGA12.1739130434782608No Hit
CAGTACAAAACACCCCACGTGCCCCCTTTTGGACACAAACTCAAAAGACA12.1739130434782608No Hit
TGTATGCACCACACGAAAAGAATGCTCTTTCACATGTTATTTGTGTATTG12.1739130434782608No Hit
ACATAAATATTTACACACCCAGCATGCAGCAACTAAATATGTAGAGCATA12.1739130434782608No Hit
CAAGTAGATCCAGACTCAGTTCTTGTCTAGTCTCTTCTGGAACTACATGC12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)