FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052662
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC64840.1283283940227943No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT63560.12579507594214695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT6400.036.256241
AGCGAAA18000.029.8106861
TTACGTG7300.029.7996482
GCGAAAG20350.027.7934822
GTTGACA44600.027.1515351
CGAAAGC21050.026.5250193
TATACTG30650.026.2608995
TAGTCGG6350.026.2599478
ACATGGT49300.024.2691215
GCACCGT43750.023.2042246
CTACTGA56900.022.9391486
AGCACCG44550.022.624775
ACCGTGT47250.022.4022038
TAATCGT2653.7844857E-621.8870949
CGTAGAC3406.4321284E-821.3274123
GACATGG55550.021.27754
CACCGTG52950.021.2229357
ATAGCAC56050.020.958323
TACGGGG5900.020.8899127
TGACATG57350.020.8625583
Produced by FastQC (version 0.11.5)