FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1805_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1805_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392090
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC56910.12957384753044676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG12750.041.514862
AGCGAAA14600.036.2548261
CGAAAGC17550.030.9870343
TCTATAC39350.028.9301573
TATACCA51450.028.7501835
ACTCCGC8350.026.0481138
CTATACC44850.025.382424
GTTCTAT41450.025.0153921
TATATAC24100.021.3617673
CTCCGAC4901.8189894E-1120.713978145
TATACTG29450.020.6818735
TTCTATA53750.020.6397232
TATATCG5103.274181E-1119.9046125
TACCACT50800.019.8382077
AGACGGT15000.019.333046145
CCGTTCA7400.018.6152259
GCTATAT19100.018.6019541
ATATACC22400.018.4511184
AGACGGC10650.018.380009145
ATACCAC58900.017.727526
Produced by FastQC (version 0.11.5)